RT info:eu-repo/semantics/article
T1 Whole Resistome Analysis in Campylobacter jejuni and C. coli Genomes Available in Public Repositories
A1 Cobo Díaz, José Francisco
A1 González del Río, Paloma
A1 Álvarez Ordóñez, Avelino
A2 Algebra
K1 Genética
K1 Campylobacter coli
K1 Resistome
K1 Whole genome sequencing
K1 Host specialization
K1 Livestock and human sources
AB Campylobacter spp. are the most frequent agent of human gastroenteritis worldwide,and the spread of multidrug-resistant strains makes the clinical treatment difficult. Thecurrent study presents the resistome analysis of 39,798 Campylobacter jejuni and11,920 Campylobacter coli genomes available in public repositories. Determinants ofresistance to β-lactams (Be) and tetracyclines (Te) were the most frequent for bothspecies, with resistance to quinolones (Qu) as the third most important on C. jejuniand to aminoglycosides (Am) on C. coli. Moreover, resistance to Te, Qu, and Amwas frequently found in co-occurrence with resistance to other antibiotic families.Geographical differences on clonal complexes distribution were found for C. jejuni andon resistome genotypes for both C. jejuni and C. coli species. Attending to the resistomepatterns by isolation source, three main clusters of genomes were found on C. jejunigenomes at antimicrobial resistance gene level. The first cluster was formed by genomesfrom human, food production animals (e.g., sheep, cow, and chicken), and food (e.g.,dairy products) isolates. The higher incidence of tet(O), associated with tetracyclineresistance, and the gyrA (T86I) single-nucleotide polymorphism (SNP), associated withquinolone resistance, among genomes from this cluster could be due to the intense useof these antibiotics in veterinary and human clinical settings. Similarly, a high incidenceof tet(O) genes of C. coli genomes from pig, cow, and turkey was found. Moreover, thecluster based on resistome patterns formed by C. jejuni and C. coli genomes of human,turkey, and chicken origin is in agreement with previous observations reporting chickenor poultry-related environments as the main source of human campylobacteriosisinfections. Most clonal complexes (CCs) associated with chicken host specialization(e.g., ST-354, ST-573, ST-464, and ST-446) were the CCs with the highest prevalenceof determinants of resistance to Be, Qu, and Te. Finally, a clear trend toward an increasein the occurrence of Te and Qu resistance determinants on C. jejuni, linked to the spreadof the co-occurrence of the bla OXA−61 and tet(O)-tet(O/W/O) genes and the gyrA (T86I)SNP, was found from 2001 to date in Europe.
LK http://hdl.handle.net/10612/13371
UL http://hdl.handle.net/10612/13371
NO -
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 03-may-2024