RT info:eu-repo/semantics/article
T1 Characterization of ESBL-producing Escherichia spp. and report of an mcr-1 colistin-resistance Escherichia fergusonni strain from minced meat in Pamplona, Colombia
A1 Martins, Joana C.L.
A1 Pintor-Cora, Alberto
A1 Alegría, Ángel
A1 Santos Buelga, Jesús Ángel
A1 Herrera-Arias, Fanny
A2 Nutricion y Bromatologia
K1 Tecnología de los alimentos
K1 Minced meat
K1 Extended-spectrum β-lactamases
K1 Colistin resistance
K1 Escherichia fergusonii
AB [EN] Foods of animal origin are increasingly considered a source of extended spectrum β-lactamase (ESBL) producing bacteria which can disseminate throughout the food chain and become a health concern for humans. This work aimed to evaluate the occurrence of ESBL-producing Escherichia coli in 100 retail minced meat samples taken in markets in Pamplona, Colombia. A total of 19 ESBL-producing isolates were obtained, 18 identified as E. coli and one as E. fergusonii. Fifteen isolates (78.9 %) carried blaCTX-M and blaTEM genes, one (5.2 %) blaSHV and blaTEM genes, one isolate (5.2 %) carried blaCTX-M and one (5.2 %) blaSHV alone. The majority of CTX-M-positive E. coli isolates carried the blaCTX-M-15 gene (13 isolates), being the blaCTX-M-9, blaCTX-M-2, and blaCTX-M-8 (one isolate each) also detected. Two SHV-positive isolates presented the blaSHV-5 and blaSHV-12 allele. The isolate identified as E. fergusonii was positive for blaCTX-M-65 gene and mcr-1 gene. Sixteen isolates (84.2 %) belonged to phylogroups A and B1 and grouped together in the phylogenetic tree obtained by MLST; phylogroups E and F were also detected. Transfer of ESBL resistance was demonstrated for the E. fergusonii isolate. Whole genome sequencing of this isolate revealed the presence of plasmids carrying additional resistance genes. This investigation showed the high prevalence of ESBL-producing E. coli in retail samples of minced meat. Also, the isolation of a strain of E. fergusonii is an additional concern, as some resistance genes are located in mobile elements, which can be transmitted to other bacteria. These evidences support the increasing public health concern considering the spreading of resistance genes through the food chain.
PB Elsevier
SN 0168-1605
LK http://hdl.handle.net/10612/15780
UL http://hdl.handle.net/10612/15780
NO Martins, J. C., Pintor-Cora, A., Alegría, Á., Santos, J. A., & Herrera-Arias, F. (2023). Characterization of ESBL-producing Escherichia spp. and report of an mcr-1 colistin-resistance Escherichia fergusonni strain from minced meat in Pamplona, Colombia. International Journal of Food Microbiology, 110168.
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 28-abr-2024