RT info:eu-repo/semantics/article
T1 Estimation by flow cytometry of percentages of survival of Listeria monocytogenes cells treated with tetracycline, with or without prior exposure to several biocides
A1 Rodríguez-Melcón, Cristina
A1 Serrano-Galán, Víctor
A1 Capita González, Rosa María
A1 Alonso Calleja, Carlos
A2 Tecnologia de los Alimentos
K1 Tecnología de los alimentos
K1 Listeria monocytogenes
K1 Antibiotic resistance
K1 Disinfectants
K1 Flow cytometry
AB [EN] In certain circumstances, disinfectants are used at sublethal concentrations. The aim of this research work was to determine whether contact of Listeria monocytogenes NCTC 11994 with subinhibitory concentrations of three disinfectants widely used in food processing environments and in the health-care system, benzalkonium chloride (BZK), sodium hypochlorite (SHY) and peracetic acid (PAA), can cause the adaptation of the strain to the biocides and increase its resistance to tetracycline (TE). The minimum inhibitory concentrations (MIC; ppm) were 2.0 (BZK), 3500.0 (SHY) and 1050.0 (PAA). On exposure to increasing subinhibitory concentrations of the biocides, the maximum concentrations (ppm) of the compounds that allowed the strain to grow were (ppm) 8.5 (BZK), 3935.5 (SHY) and 1125.0 (PAA). Both the control cells (non-exposed) and the cells that had been in contact with low doses of biocides were treated with different concentrations of TE (0 ppm, 250 ppm, 500 ppm, 750 ppm, 1000 ppm and 1250 ppm) for 24, 48 and 72 h, and the survival percentages determined using flow cytometry, following dying with SYTO 9 and propidium iodide. The cells previously exposed to PAA presented higher survival percentages (P < 0.05) than the rest of the cells for most of the concentrations of TE and treatment times trialled. These results are worrying because TE is sometimes used to treat listeriosis, highlighting the importance of avoiding the use of disinfectant at subinhibitory doses. Furthermore, the findings suggest that flow cytometry is a fast and simple technique to obtain quantitative data on bacterial resistance to antibiotics.
PB Elsevier
SN 0740-0020
LK http://hdl.handle.net/10612/15861
UL http://hdl.handle.net/10612/15861
NO Rodríguez-Melcón, C., Serrano-Galán, V., Capita, R., & Alonso-Calleja, C. (2023). Estimation by flow cytometry of percentages of survival of Listeria monocytogenes cells treated with tetracycline, with or without prior exposure to several biocides. Food Microbiology, 112(104210), 104210. https://doi.org/10.1016/j.fm.2022.10421
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 04-may-2024