RT info:eu-repo/semantics/article
T1 Effect of low sperm quality on progeny: a study on zebrafish as model species
A1 Fernández Riesco, Marta
A1 García Valcarce, David
A1 Martínez-Vázquez, Juan Manuel
A1 Robles Rodríguez, Vanesa
A2 Bioquimica y Biologia Molecular
K1 Biología
K1 Progeny
K1 Sperm
K1 Zebrafish
K1 2415 Biología Molecular
K1 2401.19 Zoología Marina
AB [EN] Nowadays a decrease tendency in human sperm quality has been reported mainly in developedcountries. Reproductive technologies have been very valuable in achieving successful pregnancieswith low quality sperm samples. However, considering that spermatozoa molecular contribution isincreasingly important in recent studies, it is crucial to study whether fertilization with low spermquality could leave a molecular mark on progeny. This study explores the consequences that fertilizationwith low sperm quality may have on progeny, using zebrafish as a model. Good and bad breeders wereestablished attending to sperm quality analyses and were individually tracked. Significant differencesin fertilization and malformation rates were obtained in progenies between high and low qualitysperm samples. Moreover an altered miR profile was found in the progenies of bad zebrafish breeders(upregulation of miR-141 and miR -122 in 24 hpf embryos) and as a consequence, some of their targetsinvolved in male sex development such as dmrt1, suffered downregulation. Our results indicatethat fertilizing with high sperm quality samples becomes relevant from a new perspective: to avoidmolecular alterations in the progeny that could remain masked and therefore produce unexpectedconsequences in it.
PB Springer
LK https://hdl.handle.net/10612/17466
UL https://hdl.handle.net/10612/17466
NO Riesco, M., Valcarce, D., Martínez Vázquez, J., Robles, V. (2019). Effect of low sperm quality on progeny: a study on zebrafish as model species. Scientific Reports. 9. 10.1038/s41598-019-47702-7
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 19-may-2024