RT info:eu-repo/semantics/article
T1 Two proteins with ornithine acetyltransferase activity show different functions in Streptomyces clavuligerus: Oat2 modulates clavulanic acid biosynthesis in response to arginine
A1 Fuente, Álvaro de la
A1 Martín Martín, Juan Francisco
A1 Rodríguez García, Antonio
A1 Liras Padín, Paloma
A2 Microbiologia
K1 Biotecnología
K1 Ornithine N-acetyltransferase
K1 Streptomyces clavuligerus
K1 Clavulanic acid biosynthesis
K1 Arginine regulation
K1 ArgR
K1 Oat2
K1 2414.03 Metabolismo Bacteriano
K1 2415.01 Biología Molecular de Microorganismos
AB [EN] The oat2 gene, located in the clavulanic acid gene cluster in Streptomyces clavuligerus, is similar to argJ, which encodes N-acetylornithine:glutamic acid acetyltransferase activity. Purified proteins obtained by expression in Escherichia coli of the argJ and oat2 genes of S. clavuligerus posses N-acetyltransferase activity. The kinetics and substrate specificities of both proteins are very similar. Deletion of the oat2 gene did not affect the total N-acetylornithine transferase activity and slightly reduced the formation of clavulanic acid under standard culture conditions. However, the oat2 mutant produced more clavulanic acid than the parental strain in cultures supplemented with high levels (above 1 mM) of arginine. The purified S. clavuligerus ArgR protein bound the arginine box in the oat2 promoter, and the expression of oat2 was higher in mutants with a disruption in argR (arginine-deregulated), confirming that the Arg boxes of oat2 are functional in vivo. Our results suggest that the Oat2 protein or one of its reaction products has a regulatory role that modulates clavulanic acid biosynthesis in response to high arginine concentrations
PB American Society for Microbiology
SN 0021-9193
LK https://hdl.handle.net/10612/17721
UL https://hdl.handle.net/10612/17721
NO Fuente, A. de la, Martín, J. F., Rodríguez-García, A. & Liras, P. (2004). Two proteins with ornithine acetyltransferase activity show different functions in Streptomyces clavuligerus: Oat2 modulates clavulanic acid biosynthesis in response to arginine. Journal of Bacteriology, 186(19), 6501-6507. https://doi.org/10.1128/JB.186.19.6501-6507.2004
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 20-may-2024