RT info:eu-repo/semantics/article
T1 Characterization of a two-gene operon epeRA involved in multidrug resistance in Streptomyces clavuligerus
A1 Rodríguez García, Antonio
A1 Santamarta, Irene
A1 Pérez Redondo, Rosario
A1 Martín Martín, Juan Francisco
A1 Liras Padín, Paloma
A2 Microbiologia
K1 Biología
K1 Multidrug resistance
K1 Streptomyces clavuligerus
K1 PqrA homolog
K1 EpeR
K1 EpeA
K1 PqrB homolog
K1 Ethidium bromide resistance
K1 Proflavine resistance
K1 2414.02 Fisiología Bacteriana
K1 2415.01 Biología Molecular de Microorganismos
AB [EN] Two genes, epeR and epeA, are located downstream of argH in the Streptomyces clavuligerus genome. EpeR belongs to the TetR family of transcriptional regulators. It is homologous to PqrA of Streptomyces coelicolor (74.3% identity) and to NfxB of Pseudomonas aeruginosa (30.9% identity). EpeA encodes a protein with 14 transmembrane spanning domains (TMS) of the major facilitator superfamily. It shares 68.9% identity to PqrB of S. coelicolor and 46.5% identity to LfrA, conferring resistance to fluoroquinolones in Mycobacterium smegmatis. Disruption of epeR results in a S. clavuligerus epeR::aph mutant which shows increased resistance to ethidium bromide and proflavine (16- and 32-fold higher than the wild type). Taking into consideration the sensitivity to drugs of different transformants carrying functional copies of either epeR or epeA, it might be concluded that both genes appear to be co-transcribed, with epeR encoding a regulatory protein which controls the expression of epeA
PB Elsevier
SN 0923-2508
LK https://hdl.handle.net/10612/17777
UL https://hdl.handle.net/10612/17777
NO Rodríguez-García, A., Santamarta, I., Pérez-Redondo, R., Martín, J. F., & Liras, P. (2006). Characterization of a two-gene operon epeRA involved in multidrug resistance in Streptomyces clavuligerus. Research in Microbiology, 157(6), 559-568. https://doi.org/10.1016/J.RESMIC.2005.12.008
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 19-may-2024