RT info:eu-repo/semantics/article
T1 Axenic interspecies and intraclonal hybrid formation in Leishmania: Successful crossings between visceral and cutaneous strains
A1 Gutiérrez Corbo, María del Camino
A1 Domínguez Asenjo, Bárbara
A1 Pérez Pertejo, Maria Yolanda
A1 García Estrada, Carlos
A1 Bello, Felio J.
A1 Balaña Fouce, Rafael
A1 Reguera Torres, Rosa María
A2 Toxicologia
K1 Medicina. Salud
K1 Leishmania
K1 Intraclonal hybrids
K1 Interspecies hybrids
K1 3109 Ciencias Veterinarias
K1 32 Ciencias Médicas
AB [EN] Neglected Tropical Diseases (NTDs) represent a serious threat to humans, especially for those living in poor or developing countries. Leishmanianiosis is considered a zoonotic NTD transmitted by the bite of female phlebotomine sandflies, and is manifested mainly as a visceral form (caused by L. infantum and L. donovani) and a cutaneous form (caused by many species including L. major, L. tropica and L. braziliensis). Although it is now known that sexual reproduction occurs in these parasites, more studies are necessary to characterize the ability of Leishmania to generate hybrids, which may represent an important mechanism for virulence, drug resistance or adaptation to the host immune system. Therefore, several experiments were conducted to generate either intraclonal or interspecies hybrids in vitro. Results demonstrated that hybrids can be formed even with outcrosses between parasites causing visceral and cutaneous forms of the disease. Characterization of hybrids in terms of ploidy, kDNA content, growth rate and infection capacity provide important information about sexual reproduction in these parasites.
PB Public Library of Science
SN 1935-2735
LK https://hdl.handle.net/10612/17828
UL https://hdl.handle.net/10612/17828
NO Gutiérrez-Corbo, C., Domínguez-Asenjo, B., Pérez-Pertejo, Y., García-Estrada, C., Bello, F. J., Balaña-Fouce, R., & Reguera, R. M. (2022). Axenic interspecies and intraclonal hybrid formation in Leishmania: Successful crossings between visceral and cutaneous strains. PLoS neglected tropical diseases, 16(2), e0010170. https://doi.org/10.1371/JOURNAL.PNTD.0010170
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 19-may-2024