RT info:eu-repo/semantics/article
T1 Metabolic switches and adaptations deduced from the proteomes of Streptomyces coelicolor wild type and phoP mutant grown in batch culture
A1 Thomas, Louise
A1 Hodgson, David A.
A1 Wentzel, Alexander
A1 Nieselt, Kay
A1 Ellingsen, Trond E.
A1 Moore, Jonathan
A1 Morrissey, Edward R.
A1 Legaie, Roxane
A1 Wohlleben, Wolfgang
A1 Rodríguez García, Antonio
A1 Martín Martín, Juan Francisco
A1 Burroughs, Nigel J.
A1 Wellington, Elizabeth M.H.
A1 Smith, Margaret C. M.
A2 Microbiologia
K1 Biología
K1 Biotecnología
K1 Metabolic switch
K1 Phosphate regulation
K1 PhoR-PhoP
K1 Streptomyces coelicolor
K1 Proteomics
K1 Antibotics biosynthesis regulation
K1 2415.01 Biología Molecular de Microorganismos
K1 2414.02 Fisiología Bacteriana
AB [EN] Bacteria in the genus Streptomyces are soil-dwelling oligotrophs and important producers of secondary metabolites. Previously, we showed that global messenger RNA expression was subject to a series of metabolic and regulatory switches during the lifetime of a fermentor batch culture of Streptomyces coelicolor M145. Here we analyze the proteome from eight time points from the same fermentor culture and, because phosphate availability is an important regulator of secondary metabolite production, compare this to the proteome of a similar time course from an S. coelicolor mutant, INB201 (ΔphoP), defective in the control of phosphate utilization. The proteomes provide a detailed view of enzymes involved in central carbon and nitrogen metabolism. Trends in protein expression over the time courses were deduced from a protein abundance index, which also revealed the importance of stress pathway proteins in both cultures. As expected, the ΔphoP mutant was deficient in expression of PhoP-dependent genes, and several putatively compensatory metabolic and regulatory pathways for phosphate scavenging were detected. Notably there is a succession of switches that coordinately induce the production of enzymes for five different secondary metabolite biosynthesis pathways over the course of the batch cultures
PB American Society for Biochemistry and Molecular Biology
SN 1535-9476
LK https://hdl.handle.net/10612/17940
UL https://hdl.handle.net/10612/17940
NO Thomas, L., Hodgson, D. A., Wentzel, A., Nieselt, K., Ellingsen, T. E., Moore, J., Morrissey, E. R., Legaie, R., Wohlleben, W., Rodríguez-García, A., Martín, J. F., Burroughs, N. J., Wellington, E. M. H., & Smith, M. C. M. (2012). Metabolic switches and adaptations deduced from the proteomes of Streptomyces coelicolor wild type and phoP mutant grown in batch culture Molecular and Cellular Proteomics, 11(2), Article eM111.013797. https://doi.org/10.1074/MCP.M111.013797
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 21-may-2024