RT info:eu-repo/semantics/article
T1 ArgR of Streptomyces coelicolor is a versatile regulator
A1 Pérez Redondo, Rosario
A1 Rodríguez García, Antonio
A1 Botas Muñoz, Alma María
A1 Santamarta, Irene
A1 Martín Martín, Juan Francisco
A1 Liras Padín, Paloma
A2 Microbiologia
K1 Biología
K1 Streptomyces coelicolor
K1 ArgR
K1 EMSA
K1 Transcriptomics
K1 Antibotics biosynthesis regulation
K1 Arginine regulation
K1 2414.02 Fisiología Bacteriana
K1 2415.01 Biología Molecular de Microorganismos
AB [EN] ArgR is the regulator of arginine biosynthesis genes in Streptomyces species. Transcriptomic comparison by microarrays has been made between Streptomyces coelicolor M145 and its mutant S. coelicolor ΔargR under control, unsupplemented conditions, and in the presence of arginine. Expression of 459 genes was different in transcriptomic assays, but only 27 genes were affected by arginine supplementation. Arginine and pyrimidine biosynthesis genes were derepressed by the lack of ArgR, while no strong effect on expression resulted on arginine supplementation. Several nitrogen metabolism genes expression as glnK, glnA and glnII, were downregulated in S. coelicolor ΔargR. In addition, downregulation of genes for the yellow type I polyketide CPK antibiotic and for the antibiotic regulatory genes afsS and scbR was observed. The transcriptomic data were validated by either reverse transcription-PCR, expression of the gene-promoter coupled to the luciferase gene, proteomic or by electrophoresis mobility shift assay (EMSA) using pure Strep-tagged ArgR. Two ARG-boxes in the arginine operon genes suggest that these genes are more tightly controlled. Other genes, including genes encoding regulatory proteins, possess a DNA sequence formed by a single ARG-box which responds to ArgR, as validated by EMSA
PB Public Library of Science
LK https://hdl.handle.net/10612/17979
UL https://hdl.handle.net/10612/17979
NO Pérez-Redondo, R., Rodríguez-García, A., Botas, A., Santamarta, I., Martín, J. F., & Liras, P. (2012). ArgR of Streptomyces coelicolor is a versatile regulator. PLoS ONE, 7(3), Article e32697. https://doi.org/10.1371/JOURNAL.PONE.0032697
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 17-may-2024