RT info:eu-repo/semantics/article T1 Characterization of Leptoglossus occidentalis Eggs and Egg Glue A1 Sánchez Hernández, Eva A1 Martín Ramos, Pablo A1 Niño Sánchez, Jonatan A1 Diez Hermano, Sergio A1 Álvarez Taboada, María Flor A1 Pérez García, Rodrigo A1 Santiago Aliste, Alberto A1 Martín Gil, Jesús A1 Diez Casero, Julio Javier A2 Ingenieria Agroforestal K1 Ingeniería forestal K1 Eggs K1 GC–MS K1 FTIR K1 Glue K1 Semiochemicals K1 SEM-EDS K1 Oviposition K1 Western conifer seed bug K1 WCSB K1 Pine nuts K1 Edible pine AB [EN] The western conifer seed bug (Leptoglossus occidentalis Heidemann, 1910, Heteroptera: Coreidae) has a significant economic impact due to the reduction in the quality and viability of conifer seed crops; it can feed on up to 40 different species of conifers, showing a clear predilection for Pinus pinea L. in Europe. Its incidence is especially relevant for the pine nut-producing industry, given that the action of this pest insect can reduce the production of pine nuts by up to 25%. As part of ongoing efforts aimed at the design of control strategies for this insect, this work focuses on the characterization (by scanning electron microscopy–energy-dispersive X-ray spectroscopy, Fourier-transform infrared spectroscopy, and gas chromatography–mass spectroscopy, GC–MS) of the compounds released by these insects during oviposition, with emphasis on the adhesive secretion that holds L. occidentalis eggs together. Elemental analysis pointed to the presence of significant amounts of compounds with high nitrogen content. Functional groups identified by infrared spectroscopy were compatible with the presence of chitin, scleroproteins, LNSP-like and gelatin proteins, shellac wax analogs, and policosanol. Regarding the chemical species identified by GC–MS, eggs and glue hydromethanolic extracts shared constituents such as butyl citrate, dibutyl itaconate, tributyl aconitate, oleic acid, oleamide, erucamide, and palmitic acid, while eggs also showed stearic and linoleic acid-related compounds. Knowledge of this composition may allow advances in new strategies to address the problem caused by L. occidentalis. PB MDPI LK https://hdl.handle.net/10612/18435 UL https://hdl.handle.net/10612/18435 NO Sánchez Hernandez, E., Martín Ramos, P., Niño Sánchez, J., Diez Hermano, S., Álvarez Taboada, F., Pérez Garcia, R., Santiago Aliste, A., Martín Gil, J. y Diez Casero, J. J. (2023). Characterization of Leptoglossus occidentalis Eggs and Egg Glue, 14(4), 396. https://doi.org/10.3390/insects14040396 DS BULERIA. Repositorio Institucional de la Universidad de León RD 19-may-2024