RT info:eu-repo/semantics/article
T1 Metagenomic comparison of the faecal and environmental resistome on Irish commercial pig farms with and without zinc oxide and antimicrobial usage
A1 Ekhlas, Daniel
A1 Cobo Díaz, José Francisco
A1 Cabrera Rubio, Raúl
A1 Alexa, Elena
A1 Ortiz Sanjuán, Juan M.
A1 García Manzanilla, Edgar
A1 Crispie, Fiona
A1 Cotter, Paul D.
A1 Leonard, Finola C.
A1 Argüello Rodríguez, Héctor
A1 Burgess, Catherine M.
A2 Tecnologia de los Alimentos
K1 Sanidad animal
K1 Antimicrobial resistance
K1 Swine
K1 Heavy metals
K1 Zinc oxide
K1 Metagenomics
K1 Resistome
K1 3109 Ciencias Veterinarias
AB [EN] Background: Antimicrobials and heavy metals such as zinc oxide (ZnO) have been commonly used on Irish commercial pig farms for a 2-week period post-weaning to help prevent infection. In 2022, the prophylactic use of antimicrobials and ZnO was banned within the European Union due to concerns associated with the emergence of antimicrobial resistance (AMR) and contamination of the environment with heavy metals. In this study, faecal and environmental samples were taken from piglets during the weaning period from ten commercial farms, of which five farms used antimicrobial or ZnO prophylaxis (AB-ZnO farms) and five which had not used antimicrobials or ZnO for the previous 3 years (AB-ZnO free farms). A total of 50 samples were compared using a metagenomic approach. Results: The results of this study showed some significant differences between AB-ZnO and AB-ZnO free farms and suggested positive selection for AMR under antimicrobial and ZnO treatment. Moreover, strong differences between environmental and faecal samples on farms were observed, suggesting that the microbiome and its associated mobile genetic elements may play a key role in the composition of the resistome. Additionally, the age of piglets affected the resistome composition, potentially associated with changes in the microbiome post-weaning. Conclusions: Overall, our study showed few differences in the resistome of the pig and its environment when comparing AB-ZnO farms with AB-ZnO free farms. These results suggest that although 3 years of removal of in-feed antimicrobial and ZnO may allow a reduction of AMR prevalence on AB-ZnO farms, more time, repeated sampling and a greater understanding of factors impacting AMR prevalence will be required to ensure significant and persistent change in on-farm AMR
PB BMC
LK https://hdl.handle.net/10612/18600
UL https://hdl.handle.net/10612/18600
NO Ekhlas, D., Cobo Díaz, J. F., Cabrera-Rubio, R., Alexa, E., Ortiz Sanjuán, J. M., Garcia Manzanilla, E., Crispie, F., Cotter, P. D., Leonard, F. C., Argüello, H., & Burgess, C. M. (2023). Metagenomic comparison of the faecal and environmental resistome on Irish commercial pig farms with and without zinc oxide and antimicrobial usage. Animal Microbiome, 5(1). https://doi.org/10.1186/S42523-023-00283-3
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 21-may-2024