RT info:eu-repo/semantics/article
T1 Comparative Proteome Analysis of Cryopreserved Flagella and Head Plasma Membrane Proteins from Sea Bream Spermatozoa: Effect of Antifreeze Proteins
A1 Zilli, Loredana
A1 Beirão Santos, José
A1 Schiavone, Roberta
A1 Herráez Ortega, María Paz
A1 Gnoni, Antonio
A1 Vilella, Sebastiano
A2 Biologia Celular
K1 Biología
K1 Membrane proteins
K1 Antifreeze proteins
K1 Sea bream spermatozoa
K1 Cryopreservation
K1 Flagella
K1 Fish sperm
K1 2407 Biología Celular
K1 3104.11 Reproducción
AB [EN] Cryopreservation induces injuries to fish spermatozoa that in turn affect sperm quality in terms of fertilization ability, motility, DNA and protein integrity and larval survival. To reduce the loss of sperm quality due to freezing-thawing, it is necessary to improve these procedures. In the present study we investigated the ability of two antifreeze proteins (AFPI and AFPIII) to reduce the loss of quality of sea bream spermatozoa due to cryopreservation. To do so, we compared viability, motility, straight-line velocity and curvilinear velocity of fresh and (AFPs)-cryopreserved spermatozoa. AFPIII addition to cryopreservation medium improved viability, motility and straight-line velocity with respect to DMSO or DMSO plus AFPI. To clarify the molecular mechanism(s) underlying these findings, the protein profile of two different cryopreserved sperm domains, flagella and head plasma membranes, was analysed. The protein profiles differed between fresh and frozen-thawed semen and results of the image analysis demonstrated that, after cryopreservation, out of 270 proteins 12 were decreased and 7 were increased in isolated flagella, and out of 150 proteins 6 showed a significant decrease and 4 showed a significant increase in head membranes. Mass spectrometry analysis identified 6 proteins (4 from isolated flagella and 2 present both in flagella and head plasma membranes) within the protein spots affected by the freezing-thawing procedure. 3 out of 4 proteins from isolated flagella were involved in the sperm bioenergetic system. Our results indicate that the ability of AFPIII to protect sea bream sperm quality can be, at least in part, ascribed to reducing changes in the sperm protein profile occurring during the freezing-thawing procedure. Our results clearly demonstrated that AFPIII addition to cryopreservation medium improved the protection against freezing respect to DMSO or DMSO plus AFPI. In addition we propose specific proteins of spermatozoa as markers related to the procedures of fish sperm cryopreservation
PB Public Library of Science (PLoS)
LK https://hdl.handle.net/10612/18710
UL https://hdl.handle.net/10612/18710
NO Zilli, L., Beirão, J., Schiavone, R., Herraez, M. P., Gnoni, A., and Vilella, S. (2014). Comparative proteome analysis of cryopreserved flagella and head plasma membrane proteins from sea bream spermatozoa: effect of antifreeze proteins. PloS One, 9(6), Article e99992. https://doi.org/10.1371/journal.pone.0099992
NO Palabras clave extraídas del título
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 17-may-2024