RT info:eu-repo/semantics/article
T1 TbpBY167A-Based Vaccine Can Protect Pigs against Glässer’s Disease Triggered by Glaesserella parasuis SV7 Expressing TbpB Cluster I
A1 Ramos Prigol, Simone
A1 Klein, Rafaela
A1 Chaudhuri, Somshukla
A1 Paraboni Frandoloso, Gabriela
A1 Guizzo, João Antônio
A1 Gutiérrez Martín, César Bernardo
A1 Schryvers, Anthony Bernard
A1 Kreutz, Luiz Carlos
A1 Frandoloso, Rafael
A2 Algebra
K1 Sanidad animal
K1 Glaesserella parasuis
K1 Glässer’s disease
K1 Serovar 7
K1 Vaccine
K1 TbpBY167A
K1 Cross-protection
K1 3109 Ciencias Veterinarias
AB [EN] Glaesserella parasuis is the etiological agent of Glässer’s disease (GD), one of the most important diseases afflicting pigs in the nursery phase. We analyzed the genetic and immunological properties of the TbpB protein naturally expressed by 27 different clinical isolates of G. parasuis that were typed as serovar 7 and isolated from pigs suffering from GD. All the strains were classified as virulent by LS-PCR. The phylogenetic analyses demonstrated high similarity within the amino acid sequence of TbpB from 24 clinical strains all belonging to cluster III of TbpB, as does the protective antigen TbpBY167A. Three G. parasuis isolates expressed cluster I TbpBs, indicating antigenic diversity within the SV7 group of G. parasuis. The antigenic analysis demonstrated the presence of common epitopes on all variants of the TbpB protein, which could be recognized by an in vitro analysis using pig IgG induced by a TbpBY167A-based vaccine. The proof of concept of the complete cross-protection between clusters I and III was performed in SPF pigs immunized with the TbpBY167A-based vaccine (cluster III) and challenged with G. parasuis SV7, strains LM 360.18 (cluster I). Additionally, pigs immunized with a whole-cell inactivated vaccine based on G. parasuis SV5 (Nagasaki strain) did not survive the challenge performed with SV7 (strain 360.18), demonstrating the absence of cross-protection between these two serovars. Based on these results, we propose that a properly formulated TbpBY167A-based vaccine may elicit a protective antibody response against all strains of G. parasuis SV7, despite TbpB antigenic diversity, and this might be extrapolated to other serovars. This result highlights the promising use of the TbpBY167A antigen in a future commercial vaccine for GD prevention.
PB MDPI
LK https://hdl.handle.net/10612/18835
UL https://hdl.handle.net/10612/18835
NO Prigol, S. R., Klein, R., Chaudhuri, S., Frandoloso, G. P., Guizzo, J. A., Martín, C. B. G., Schryvers, A. B., Kreutz, L. C., & Frandoloso, R. (2022). TbpBY167A-Based Vaccine Can Protect Pigs against Glässer’s Disease Triggered by Glaesserella parasuis SV7 Expressing TbpB Cluster I. Pathogens, 11(7). https://doi.org/10.3390/PATHOGENS11070766
NO Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/).
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 16-may-2024