RT info:eu-repo/semantics/article
T1 Molecular serotyping of clinical strains of Haemophilus (Glaesserella) parasuis brings new insights regarding Glässer’s disease outbreaks in Brazil
A1 Pires Espíndola, Julia
A1 Balbinott, Natalia
A1 Gressler, Letícia Trevisan
A1 Machado, Gustavo
A1 Klein, Catia Silene
A1 Rebelatto, Raquel
A1 Gutiérrez Martín, César Bernardo
A1 Kreutz, Luiz Carlos
A1 Schryvers, Anthony Bernard
A1 Frandoloso, Rafael
A2 Sanidad Animal
K1 Veterinaria
K1 Disease mapping
K1 Haemophilus parasuis
K1 Typification
K1 Serovars
K1 Vaccines
K1 Brazil
K1 3109 Ciencias Veterinarias
K1 3109.05 Microbiología
AB [EN]Glässer's disease (GD) is an important infectious disease of swine caused by Haemophilus (Glaesserella) parasuis. Vaccination with inactivated whole cell vaccines is the major approach for prevention of H. parasuis infection worldwide, but the immunity induced is predominantly against the specific polysaccharide capsule. As a consequence, the available vaccines may not induce adequate protection against the field strains, when the capsules present in the vaccine strains are different from those in strains isolated from the farms. Therefore, it is crucial to map H. parasuis serovars associated with regional outbreaks so that appropriate bacterin vaccines can be developed and distributed for prevention of infection. In this study, 459 H. parasuis field strains isolated from different Glässer's disease outbreaks that occurred in 10 different Brazilian States were analyzed for serotype using PCR-based approaches. Surprisingly, non-Typeable (NT) strains were the second most prevalent group of field strains and along with serovars 4, 5 and 1 comprised more than 70% of the isolates. A PCRbased approach designed to amplify the entire polysaccharide capsule locus revealed 9 different band patterns in the NT strains, and 75% of the NT strains belonged to three clusters, suggesting that a number of new serovars are responsible for a substantial proportion of disease. These results indicate that commercially available vaccines in Brazil do not cover the most prevalent H. parasuis serovars associated with GD.
PB PeerJ
LK https://hdl.handle.net/10612/19340
UL https://hdl.handle.net/10612/19340
NO Espíndola, J. P., Balbinott, N., Gressler, L. T., Machado, G., Klein, C. S., Rebelatto, R., Martín, C. B. G., Kreutz, L. C., Schryvers, A. B., & Frandoloso, R. (2019). Molecular serotyping of clinical strains of Haemophilus (Glaesserella) parasuis brings new insights regarding Glässer’s disease outbreaks in Brazil. PeerJ, 7, Article e6817. https://doi.org/10.7717/PEERJ.6817
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 21-may-2024