RT info:eu-repo/semantics/article
T1 Assessment of a plasmid conjugation procedure to monitor horizontal transfer of an extended-spectrum β-lactamase resistance gene under food chain scenarios
A1 Álvarez Molina, Adrián
A1 Trigal, Elena
A1 Prieto Maradona, Miguel
A1 López Fernández, María Mercedes
A1 Álvarez Ordóñez, Avelino
A2 Tecnologia de los Alimentos
K1 Tecnología de los alimentos
K1 Plasmid
K1 E. coli
K1 ESBL
K1 Conjugation
K1 Horizontal gene transfer
K1 3309 Tecnología de Los Alimentos
AB [EN] Plasmids are relevant reservoirs of antimicrobial resistance genes (ARGs) which confer adaptive advantages to their host and can be horizontally transferred. The aims of this study were to develop a conjugation procedure to monitor the horizontal transfer of a 193 kb plasmid containing the extended-spectrum β-lactamase production gene blaCTX-M-14 between two Escherichia coli strains under a range of food chain-related scenarios, including temperature (20–37 °C), pH (5.0–9.0) or the presence of some biocidal agents (benzalkonium chloride, sodium hypochlorite or peracetic acid). The average conjugation rate in LB broth after 18 h at 37 °C was 2.09e-04 and similar rates were observed in a food matrix (cow's milk). The conjugation was reduced at temperatures below 37 °C, at alkaline pH (especially at pH 9.0) or in the presence of benzalkonium chloride. Peracetic acid and sodium hypochlorite slightly increased conjugation rates, which reached 5.59e-04 and 6.77e-03, respectively. The conjugation procedure described can be used to identify risk scenarios leading to an enhanced ARGs transmission via plasmid conjugation, as well as to identify novel intervention strategies impairing plasmid conjugation and tackling antimicrobial resistance.
PB Elsevier
SN 2665-9271
LK https://hdl.handle.net/10612/19411
UL https://hdl.handle.net/10612/19411
NO Álvarez Molina, A., Trigal, E., Prieto, M., López, M., & Álvarez Ordóñez, A. (2023). Assessment of a plasmid conjugation procedure to monitor horizontal transfer of an extended-spectrum β-lactamase resistance gene under food chain scenarios. Current Research in Food Science, 6. https://doi.org/10.1016/J.CRFS.2022.11.024
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 17-may-2024