RT info:eu-repo/semantics/article
T1 Genomic Insights into the Mobilome and Resistome of Sentinel Microorganisms Originating from Farms of Two Different Swine Production Systems
A1 Mencía Ares, Óscar
A1 Borowiak, Maria
A1 Argüello Rodríguez, Héctor
A1 Cobo Díaz, José Francisco
A1 Malorny, Burkhard
A1 Álvarez Ordóñez, Avelino
A1 Carvajal Urueña, Ana María
A1 Deneke, Carlus
A2 Sanidad Animal
K1 Producción animal
K1 Antimicrobial resistance
K1 Campylobacter coli
K1 Escherichia coli
K1 Enterococcus spp.
K1 Mobile genetic element
K1 Pigs
K1 Staphylococcus spp.
K1 Sustainable farming
K1 Whole- genome sequencing
K1 3309 Tecnología de Los Alimentos
AB [EN] Antimicrobial resistance (AMR) is a threat to public health due to long-term antimicrobial use (AMU), which promotes the bacterial acquisition of antimicrobial resistance determinants (ARDs). Within food-producing animals, organic and extensive Iberian swine production is based on sustainable and eco-friendly management systems, providing an excellent opportunity to evaluate how sustained differences in AMU impact the development and spread of AMR. Here, through a whole-genome sequencing approach, we provide an in-depth characterization of the resistome and mobilome and their interaction in 466 sentinel bacteria, namely, Escherichia coli, Enterococcus spp., Campylobacter coli, and Staphylococcus spp., recovered from 37 intensive and organic-extensive pig farms. Both ARDs and mobile genetic elements (MGEs) were primarily taxon-associated, with higher similarities among bacteria which were closely phylogenetically related. E. coli exhibited the most diverse resistome and mobilome, with 85.4% mobilizable ARDs, 50.3% of which were plasmid-associated. Staphylococcus spp. exhibited a broad repertoire of ARDs and MGEs, with 52.3% of its resistome being mobilizable. Although Enterococcus spp. carried the highest number of ARDs per isolate and its plasmidome was similar in size to that of E. coli, 43.7% of its resistome was mobilizable. A narrow spectrum of ARDs constituted the C. coli resistome, with point mutations as its main AMR driver. A constrained AMU, as observed in organic-extensive herds, determined a reduction in the quantitative composition of the resistome and the complexity of the resistome-mobilome interaction. These results demonstrate taxon-associated AMR-MGE interactions and evidence that responsible AMU can contribute to reducing AMR pressure in the food chain
PB American Society for Microbiology
LK https://hdl.handle.net/10612/20397
UL https://hdl.handle.net/10612/20397
NO Mencía Ares, O., Borowiak, M., Argüello, H., Cobo Díaz, J. F., Malorny, B., Álvarez-Ordóñez, A., Carvajal, A., & Deneke, C. (2022). Genomic Insights into the Mobilome and Resistome of Sentinel Microorganisms Originating from Farms of Two Different Swine Production Systems. Microbiology Spectrum, 10(6). https://doi.org/10.1128/SPECTRUM.02896-22
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 02-jun-2024