RT info:eu-repo/semantics/article
T1 Study on Viability and Chondrogenic Differentiation of Cryopreserved Adipose Tissue-Derived Mesenchymal stromal Cells for future use in regenerative medicine
A1 López-González, María Elisa
A1 Pérez Castrillo, Saúl
A1 Ordás-Fernández, P.
A1 López-González, M.E.
A1 Colaço, Bruno Jorge Antunes
A1 Villar Suárez, María Vega
A2 Otros
K1 Anatomía
K1 Veterinaria
K1 Cryopreservation
K1 Crioperservación
K1 Chondrogenic differentiation
K1 Medicina regenerativa
AB Adipose-derived mesenchymal stromal cells are promising as a regenerative therapy tool for defectivetissues in mesenchymal lineage, including fat, bone, cartilage, and blood vessels. In potential future clinicalapplications, adipose-derived stem cell cryopreservation is an essential fundamental technology. Theaim of this study is to define an adequate protocol for the cryopreservation of adipose-derived mesenchymalstromal cells, by comparing various protocols so as to determine the effects of cryopreservation onviability and chondrogenic differentiation potential of adipose-derived stem cells upon freeze-thawing ofAT-MSCs colonies cryopreserved with standard and modified protocols, using flow cytometry and confocalmicroscopy. The study concludes that adipose-derived mesenchymal stromal cells could be long-termcryopreserved without any loss of their proliferative or differentiation potential
PB Elsevier
YR 2017
FD 2017-07-25
LK http://hdl.handle.net/10612/6449
UL http://hdl.handle.net/10612/6449
NO Cryobiology (2015), n. 71
NO P. 256–263
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 28-abr-2024