RT info:eu-repo/semantics/article
T1 Development and Characterization of Protective Haemophilus parasuis Subunit Vaccines Based on Native Proteins with Affinity to Porcine Transferrin and Comparison with Other Subunit and Commercial Vaccines
A1 Frandoloso, Rafael
A1 Martínez Martínez, Sonia
A1 Rodríguez Ferri, Elías Fernando
A1 García Iglesias, María José
A1 Pérez Martínez, Claudia
A1 Martínez-Fernández, Beatriz
A1 Gutiérrez Martín, César Bernardo
A2 Sanidad Animal
K1 Sanidad animal
K1 Haemophilus parasuis
K1 Commercial Vaccines
K1 Subunit Vaccines
K1 Ganado porcino
AB Haemophilus parasuis is the agent responsible for causing Glasser’s disease, which is characterized byfibrinous polyserositis, polyarthritis, and meningitis in pigs. In this study, we have characterized native outermembrane proteins with affinity to porcine transferrin (NPAPT) from H. parasuis serovar 5, Nagasaki strain.This pool of proteins was used as antigen to developed two vaccine formulations: one was adjuvanted with amineral oil (Montanide IMS 2215 VG PR), while the other was potentiated with a bacterial neuraminidasefrom Clostridium perfringens. The potential protective effect conferred by these two vaccines was compared tothat afforded by two other vaccines, consisting of recombinant transferrin-binding protein (rTbp) A or Bfragments from H. parasuis, Nagasaki strain, and by a commercially available inactivated vaccine. Five groupsof colostrum-deprived piglets immunized with the vaccines described above, one group per each vaccine, anda group of nonvaccinated control animals were challenged intratracheally with a lethal dose (3   108 CFU) ofH. parasuis, Nagasaki strain. The two vaccines containing rTbps yielded similar results with minimal protectionagainst death, clinical signs, gross and microscopic lesions, and H. parasuis invasion. In contrast, the twovaccines composed of NPAPT antigen and commercial bacterin resulted in a strong protection againstchallenge (without deaths and clinical signs), mild histopathological changes, and no recovery of H. parasuis,thus suggesting their effectiveness in preventing Glasser’s disease outbreaks caused by serovar 5
PB American Society for Microbiology
YR 2017
FD 2017-08-01
LK http://hdl.handle.net/10612/6460
UL http://hdl.handle.net/10612/6460
NO Clinical and Vaccine Immunology, January 2011, vol. 18, n. 1
NO P. 50-58
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 27-abr-2024