RT info:eu-repo/semantics/preprint
T1 The Breast Cancer Resistance Protein (BCRP/ABCG2) as a key player in the tissue distribution of flaxseed lignans and their metabolites
A1 García Mateos, Dafne
A1 García-Villalba, Rocío
A1 Otero, Jon Andoni
A1 Marañón, José Ángel
A1 Espín, Juan Carlos
A1 Álvarez de Felipe, Ana Isabel
A1 Merino Peláez, Gracia
A2 Fisiologia
K1 Fisiología
K1 Cáncer
K1 BCRP/ABCG2
K1 Proteínas
AB Lignans are dietary polyphenols, which are metabolized by the gut microbiota into thephytoestrogenic metabolites enterolignans, mainly enterolactone and enterodiol. The BreastCancer Resistance Protein (BCRP/ABCG2) is an efflux transporter that affects plasma andmilk secretion of several drugs and natural compounds. We hypothesized here that Abcg2could influence levels of lignans and their derived metabolites in target tissues.Consequently, we aimed to evaluate the role of Abcg2 in the tissue distribution of thesecompounds. We used Abcg2-/- knockout and wild-type male mice fed with a lignanenricheddiet for one week and analysed plasma, small intestine, colon, liver, kidneys andtesticles. High levels of lignans as well as enterolignans and their glucuronide and sulfateconjugates in the small intestine and colon were detected, with higher concentrations of theconjugates in the wild-type compared with Abcg2-/- mice. Particularly relevant was thedetection of 24-fold and 8-fold higher concentrations of enterolactone-sulfate andenterolactone-glucuronide, respectively, in the kidney of Abcg2-/- compared with wild-typemice. In conclusion, our study showed that lignans and their derived metabolites were invivo substrates of Abcg2, which affected their plasma and tissue levels. These resultshighlight the role of Abcg2 in influencing the health-beneficial properties of dietarylignans
PB Royal Society of Chemistry
YR 2018
FD 2018-10-15
LK http://hdl.handle.net/10612/8801
UL http://hdl.handle.net/10612/8801
NO Food & Function
NO 25 p.
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 27-abr-2024