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dc.contributor | Facultad de Veterinaria | es_ES |
dc.contributor.author | Fernández Escobar, Mercedes | |
dc.contributor.author | Calero Bernal, Rafael | |
dc.contributor.author | Benavides, Julio | |
dc.contributor.author | Regidor Cerrillo, Javier | |
dc.contributor.author | Guerrero Molina, María Cristina | |
dc.contributor.author | Gutiérrez Expósito, Daniel | |
dc.contributor.author | Collantes Fernández, Esther | |
dc.contributor.author | Ortega Mora, Luis Miguel | |
dc.contributor.other | Sanidad Animal | es_ES |
dc.date | 2020 | |
dc.date.accessioned | 2024-03-20T08:59:23Z | |
dc.date.available | 2024-03-20T08:59:23Z | |
dc.identifier.citation | Fernández-Escobar, M., Calero-Bernal, R., Benavides, J., Regidor-Cerrillo, J., Guerrero-Molina, M. C., Gutiérrez-Expósito, D., Collantes-Fernández, E., & Ortega-Mora, L. M. (2020). Isolation and genetic characterization of Toxoplasma gondii in Spanish sheep flocks. Parasites and Vectors, 13(1). https://doi.org/10.1186/S13071-020-04275-Z | es_ES |
dc.identifier.other | https://parasitesandvectors.biomedcentral.com/articles/10.1186/s13071-020-04275-z | es_ES |
dc.identifier.uri | https://hdl.handle.net/10612/19117 | |
dc.description.abstract | [EN] Background: Toxoplasma gondii is a major cause of abortion in small ruminants and presents a zoonotic risk when undercooked meat containing cysts is consumed. The aim of the present study was to investigate the genetic diversity among the T. gondii strains circulating in ovine livestock in Spain. Methods: Selected samples collected from abortion outbreaks due to toxoplasmosis (n = 31) and from chronically infected adult sheep at slaughterhouses (n = 50) in different Spanish regions were bioassayed in mice, aiming at parasite isolation. In addition, all original clinical samples and the resulting isolates were genotyped by multi-nested PCR-RFLP analysis of 11 molecular markers and by PCR-DNA sequencing of portions of the SAG3, GRA6 and GRA7 genes. Results: As a result, 30 isolates were obtained from 9 Spanish regions: 10 isolates from abortion-derived samples and 20 isolates from adult myocardial tissues. Overall, 3 genotypes were found: ToxoDB#3 (type II PRU variant) in 90% (27/30) of isolates, ToxoDB#2 (clonal type III) in 6.7% (2/30), and ToxoDB#1 (clonal type II) in 3.3% (1/30). When T. gondii-positive tissue samples (n = 151) were directly subjected to RFLP genotyping, complete restriction profiles were obtained for 33% of samples, and up to 98% of the specimens belonged to the type II PRU variant. A foetal brain showed a clonal type II pattern, and four specimens showed unexpected type I alleles at the SAG3 marker, including two foetal brains that showed I + II alleles as co-infection events. Amplicons of SAG3, GRA6 and GRA7 obtained from isolates and clinical samples were subjected to sequencing, allowing us to confirm RFLP results and to detect different single-nucleotide polymorphisms. Conclusions: The present study informed the existence of a predominant type II PRU variant genotype (ToxoDB#3) infecting domestic sheep in Spain, in both abortion cases and chronic infections in adults, coexisting with other clonal (ToxoDB#1 and ToxoDB#2), much less frequent genotypes, as well as polymorphic strains as revealed by clinical sample genotyping. The use of multilocus sequence typing aided in accurately estimating T. gondii intragenotype diversity | es_ES |
dc.language | eng | es_ES |
dc.publisher | BMC | es_ES |
dc.rights | Atribución 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject | Sanidad animal | es_ES |
dc.subject.other | Toxoplasma gondii | es_ES |
dc.subject.other | Sheep | es_ES |
dc.subject.other | Abortion | es_ES |
dc.subject.other | Isolates | es_ES |
dc.subject.other | Genotyping | es_ES |
dc.subject.other | Sequencing | es_ES |
dc.subject.other | Population structure | es_ES |
dc.subject.other | Spain | es_ES |
dc.title | Isolation and genetic characterization of Toxoplasma gondii in Spanish sheep flocks | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.identifier.doi | 10.1186/S13071-020-04275-Z | |
dc.description.peerreviewed | SI | es_ES |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es_ES |
dc.identifier.essn | 1756-3305 | |
dc.journal.title | Parasites & Vectors | es_ES |
dc.volume.number | 13 | es_ES |
dc.issue.number | 1 | es_ES |
dc.page.initial | 396 | es_ES |
dc.type.hasVersion | info:eu-repo/semantics/publishedVersion | es_ES |
dc.subject.unesco | 3109 Ciencias Veterinarias | es_ES |
dc.description.project | This research was supported by projects funded by the Spanish Ministry of Science and Innovation (AGL2016-75935-C2-R) and the Community of Madrid (PLATESA2-CM-P2018/BAA-4370). MF and RC were funded by UCMSantander/ 2017 pre-doctoral grants, and PLATESA2 post-doctoral grants, respectively. CG was funded by DGAPA, National Autonomous University of Mexico (UNAM). RC, EC and LO are part of the TOXOSOURCES consortium, supported by funding from the European Union’s Horizon 2020 Research and Innovation programme under grant agreement No. 773830: One Health European Joint Programme | es_ES |
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