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dc.contributor | Facultad de Veterinaria | es_ES |
dc.contributor.author | Ráez Bravo, Arián | |
dc.contributor.author | Granados, José Enrique | |
dc.contributor.author | Serrano, Emmanuel | |
dc.contributor.author | Dellamaria, Debora | |
dc.contributor.author | Casais, Rosa | |
dc.contributor.author | Rossi, Luca | |
dc.contributor.author | Puigdemont, Anna | |
dc.contributor.author | Cano Manuel, Francisco Javier | |
dc.contributor.author | Fandos, Paulino | |
dc.contributor.author | Pérez, Jesús María | |
dc.contributor.author | Espinosa Cerrato, José | |
dc.contributor.author | Soriguer, Ramón C. | |
dc.contributor.author | Citterio, Carlo | |
dc.contributor.author | López Olvera, Jorge Ramón | |
dc.contributor.other | Sanidad Animal | es_ES |
dc.date | 2016 | |
dc.date.accessioned | 2024-04-17T08:52:12Z | |
dc.date.available | 2024-04-17T08:52:12Z | |
dc.identifier.citation | Ráez Bravo, A., Granados, J. E., Serrano, E., Dellamaria, D., Casais, R., Rossi, L., Puigdemont, A., Cano Manuel, F. J., Fandos, P., Pérez, J. M., Espinosa, J., Soriguer, R. C., Citterio, C., & López-Olvera, J. R. (2016). Evaluation of three enzyme-linked immunosorbent assays for sarcoptic mange diagnosis and assessment in the Iberian ibex, Capra pyrenaica. Parasites and Vectors, 9(1). https://doi.org/10.1186/S13071-016-1843-4 | es_ES |
dc.identifier.uri | https://hdl.handle.net/10612/19865 | |
dc.description.abstract | [EN] Background: Sarcoptic mange is a contagious skin disease caused by the mite Sarcoptes scabiei, affecting different mammalian species worldwide including the Iberian ibex (Capra pyrenaica), in which mortalities over 90 % of the population have been reported. No efficient diagnostic methods are available for this disease, particularly when there are low mite numbers and mild or no clinical signs. In this study, three enzyme-linked immunosorbent assays (ELISA) developed for dog (ELISA A), Cantabrian chamois (Rupicapra pyrenaica parva) (ELISA B) and Alpine chamois (Rupicapra rupicapra) (ELISA C), were evaluated to detect specific antibodies (IgG) to sarcoptic mange in Iberian ibex sera. Methods: Serum samples from 131 Iberian ibexes (86 healthy and 45 scabietic) were collected from 2005 to 2012 in the Sierra Nevada Natural and National Parks (southern Spain). Based on visual inspection, ibexes were classified into one of three categories, namely healthy (without scabietic compatible lesions), mildly affected (skin lesions over less than 50 % of the body surface) and severely affected (skin lesions over more than 50 % of the body surface). The optimal cut-off point, specificity, sensitivity and the area under the curve (AUC) were calculated, and the agreement between tests was determined. Moreover, differences in the optical density (OD) related to scabies severity have been evaluated for the best test. Results: ELISA C showed better performance than the two other tests, reaching higher values of sensitivity (93.0 %) and specificity (93.5 %) against the visual estimation of the percentage of affected skin, chosen as the gold standard. Significantly higher concentrations of specific antibodies were observed with this test in the mildly and severely infested ibexes than in healthy ones. Conclusions: Our results revealed that ELISA C was an optimal test to diagnose sarcoptic mange in the Iberian ibex. Further studies characterizing immune response during the course of the disease, including spontaneous or drug induced recovery, should follow in order to better understand sarcoptic mange in Iberian ibex populations | es_ES |
dc.language | eng | es_ES |
dc.publisher | BMC | es_ES |
dc.rights | Atribución 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject | Sanidad animal | es_ES |
dc.subject.other | Capra pyrenaica | es_ES |
dc.subject.other | Diagnostic | es_ES |
dc.subject.other | ELISA | es_ES |
dc.subject.other | Sarcoptes scabiei | es_ES |
dc.subject.other | Serum | es_ES |
dc.title | Evaluation of three enzyme-linked immunosorbent assays for sarcoptic mange diagnosis and assessment in the Iberian ibex, Capra pyrenaica | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.identifier.doi | 10.1186/S13071-016-1843-4 | |
dc.description.peerreviewed | SI | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/ MINECO/ Programa Nacional de Investigación Fundamental/CGL2012-40043-C02-02 /ES/CARACTERIZACION DE LA INFESTACION EXPERIMENTAL POR SARCOPTES SCABIEI EN LA CABRA MONTES (CAPRA PYRENAICA)// | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/MINECO/ Programa Estatal de Promoción del Talento y su Empleabilidad/ BES-2013-063931/ES/ BES-2013-063931// | es_ES |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es_ES |
dc.identifier.essn | 1756-3305 | |
dc.journal.title | Parasites & Vectors | es_ES |
dc.volume.number | 9 | es_ES |
dc.issue.number | 1 | es_ES |
dc.type.hasVersion | info:eu-repo/semantics/publishedVersion | es_ES |
dc.subject.unesco | 3109 Ciencias Veterinarias | es_ES |
dc.description.project | The Consejería de Medio Ambiente y Ordenación del Territorio of the Junta de Andalucía supported sample collection and conservation through the projects 676/2006/A/00, 1571/2007/M/00, 173/2009/M/00 and 861/2011/M/ 00, as did the PAIDI Research Group RNM18 from the Junta de Andalucía. The Ministerio de Economía y Competitividad of the Spanish Government funded the study through the research projects CGL2012-40043-C02-02 and CGL2016-80543-P, and a predoctoral grant (BES-2013-063931) to JE. ES was supported by the Fundação para a Ciência ea Tecnologia (Portugal), through the postdoctoral program (SFRH/BPD/96637/2013), and the University of Aveiro (Department of Biology) and FCT/MEC (CESAM RU UID/AMB/50017), co-financed by the FEDER within the PT2020 Partnership Agreement. LR, DD and CC were supported by the Italian National PRIN Program “Genomics and host-pathogen interaction: a study model in the One-Health perspective” | es_ES |
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