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dc.contributor | Facultad de Veterinaria | es_ES |
dc.contributor.author | Silva, Vanessa | |
dc.contributor.author | Correia, Elisete | |
dc.contributor.author | Pereira, José Eduardo | |
dc.contributor.author | González Machado, Camino | |
dc.contributor.author | Capita González, Rosa María | |
dc.contributor.author | Alonso Calleja, Carlos | |
dc.contributor.author | Igrejas, Gilberto | |
dc.contributor.author | Poeta, Patrícia | |
dc.contributor.other | Nutricion y Bromatologia | es_ES |
dc.date | 2022 | |
dc.date.accessioned | 2024-05-02T08:56:19Z | |
dc.date.available | 2024-05-02T08:56:19Z | |
dc.identifier.citation | Silva, V., Correia, E., Pereira, J. E., González-Machado, C., Capita, R., Alonso-Calleja, C., Igrejas, G., & Poeta, P. (2022). Biofilm Formation of Staphylococcus aureus from Pets, Live-Stock, and Wild Animals: Relationship with Clonal Lineages and Antimicrobial Resistance. Antibiotics, 11(6) Article e772. https://doi.org/10.3390/ANTIBIOTICS11060772 | es_ES |
dc.identifier.other | https://www.mdpi.com/2079-6382/11/6/772 | es_ES |
dc.identifier.uri | https://hdl.handle.net/10612/20243 | |
dc.description | Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/). | es_ES |
dc.description.abstract | [ES] This study aimed to compare the biofilm formation ability of Staphylococcus aureus isolated from a wide range of animals and study the association between biofilm formation and antimicrobial resistance and genetic lineages. A total of 214 S. aureus strains isolated from pets, livestock, and wild animals were evaluated regarding their ability to form biofilms by the microtiter biofilm assay and their structure via confocal scanning laser microscopy. Statistical analysis was used to find an association between biofilm formation and antimicrobial resistance, multidrug resistance, sequence types (STs), spa and agr-types of the isolates. The antimicrobial susceptibility of 24 h-old biofilms was assessed against minimum inhibitory concentrations (MIC) and 10× MIC of amikacin and tetracycline, and the biomass reduction was measured. The metabolic activity of biofilms after antimicrobial treatment was evaluated by the XTT assay. All isolates were had the ability to form biofilms. Yet, significant differences in biofilm biomass production were detected among animal species. Multidrug resistance had a positive association with biofilm formation as well as methicillin-resistance. Significant differences were also detected among the clonal lineages of the isolates. Both tetracycline and amikacin were able to significantly reduce the biofilm mass. However, none of the antimicrobials were able to eradicate the biofilm at the maximum concentration used. Our results provide important information on the biofilm-forming capacity of animal-adapted S. aureus isolates, which may have potential implications for the development of new biofilm-targeted therapeutics. | es_ES |
dc.language | eng | es_ES |
dc.publisher | MDPI | es_ES |
dc.rights | Atribución 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject | Tecnología de los alimentos | es_ES |
dc.subject.other | S. aureus | es_ES |
dc.subject.other | MRSA | es_ES |
dc.subject.other | Animals | es_ES |
dc.subject.other | Biofilms | es_ES |
dc.subject.other | Antimicrobial resistance | es_ES |
dc.subject.other | Genetic linages | es_ES |
dc.title | Biofilm Formation of Staphylococcus aureus from Pets, Livestock, and Wild Animals: Relationship with Clonal Lineages and Antimicrobial Resistance | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.identifier.doi | 10.3390/ANTIBIOTICS11060772 | |
dc.description.peerreviewed | SI | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/AEI/Programa Estatal de I+D+i Orientada a los Retos de la Sociedad/RTI2018-098267-R-C33/ES/ALTERNATIVAS AL USO DE DESINFECTANTES EN LA INDUSTRIA ALIMENTARIA DIRIGIDAS A REDUCIR LA SUPERVIVENCIA DE LISTERIA MONOCYTOGENES Y SALMONELLA ENTERICA SOBRE LAS SUPERFICIES// | es_ES |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es_ES |
dc.identifier.essn | 2079-6382 | |
dc.journal.title | Antibiotics | es_ES |
dc.volume.number | 11 | es_ES |
dc.issue.number | 6 | es_ES |
dc.page.initial | 772 | es_ES |
dc.type.hasVersion | info:eu-repo/semantics/publishedVersion | es_ES |
dc.subject.unesco | 3109.05 Microbiología | es_ES |
dc.subject.unesco | 2414.04 Bacteriología | es_ES |
dc.description.project | This work was funded by the R&D Project CAREBIO2: Comparative assessment of antimicrobial resistance in environmental biofilms through proteomics—towards innovative theranostic biomarkers, with reference NORTE-01-0145-FEDER-030101 and PTDC/SAU-INF/30101/2017, financed by the European Regional Development Fund (ERDF) through the Northern Regional Operational Program (NORTE 2020) and the Foundation for Science and Technology (FCT). This work was supported by the Associate Laboratory for Green Chemistry-LAQV, which is financed by national funds from FCT/MCTES (UIDB/50006/2020 and UIDP/50006/2020) and by the projects UIDB/CVT/00772/2020 and LA/P/0059/2020 funded by the Portuguese Foundation for Science and Technology (FCT). The Ministerio de Ciencia, Innovación y Universidades (Spain, grant number RTI2018-098267-R-C33) and the Junta de Castilla y León (Consejería de Educación, Spain, grant number LE018P20). Vanessa Silva is grateful to FCT (Fundacão para a Ciência e a Tecnologia) for financial support through the PhD grant SFRH/BD/137947/2018. | es_ES |
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