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Título
Overexpression of ZePrx in Nicotiana tabacum Affects Lignin Biosynthesis Without Altering Redox Homeostasis
Autor
Facultad/Centro
Área de conocimiento
Título de la revista
Frontiers in Plant Science
Datos de la obra
García-Ulloa, A., Sanjurjo, L., Cimini, S., Encina, A., Martínez-Rubio, R., Bouza, R., Barral, L., Estévez-Pérez, G., Novo-Uzal, E., De Gara, L., & Pomar, F. (2020). Overexpression of ZePrx in Nicotiana tabacum Affects Lignin Biosynthesis Without Altering Redox Homeostasis. Frontiers in Plant Science, 11. https://doi.org/10.3389/FPLS.2020.00900
Editor
Frontiers Media
Fecha
2020
Résumé
[EN] Class III plant peroxidases (Prxs) are involved in the oxidative polymerization of lignins.
Zinnia elegans Jacq. Basic peroxidase (ZePrx) has been previously characterized as
capable of catalyzing this reaction in vitro and the role in lignin biosynthesis of several
of its Arabidopsis thaliana homologous has been previously confirmed. In the present
work, ZePrx was overexpressed in Nicotiana tabacum to further characterize its function
in planta with particular attention to its involvement in lignin biosynthesis. Since Prxs
are known to alter ROS levels by using them as electron acceptor or producing them
in their catalytic activity, the impact of this overexpression in redox homeostasis was
studied by analyzing the metabolites and enzymes of the ascorbate-glutathione cycle.
In relation to the modification induced by ZePrx overexpression in lignin composition
and cellular metabolism, the carbohydrate composition of the cell wall as well as overall
gene expression through RNA-Seq were analyzed. The obtained results indicate that
the overexpression of ZePrx caused an increase in syringyl lignin in cell wall stems,
suggesting that ZePrx is relevant for the oxidation of sinapyl alcohol during lignin
biosynthesis, coherently with its S-peroxidase nature. The increase in the glucose
content of the cell wall and the reduction of the expression of several genes involved in
secondary cell wall biosynthesis suggests the occurrence of a possible compensatory
response to maintain cell wall properties. The perturbation of cellular redox homeostasis
occurring as a consequence of ZePrx overexpression was kept under control by an
increase in APX activity and a reduction in ascorbate redox state. In conclusion, our
results confirm the role of ZePrx in lignin biosynthesis and highlight that its activity alters
cellular pathways putatively aimed at maintaining redox homeostasis.
Materia
Palabras clave
Peer review
SI
ID proyecto
- info:eu-repo/grantAgreement/MECD/ Programa Estatal de Promoción del Talento y su Empleabilidad/ FPU13/04835/ES
URI
DOI
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