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Título
Post-Thaw Sperm Quality and Functionality in the Autochthonous Pig Breed Gochu Asturcelta
Autor
Facultad/Centro
Área de conocimiento
Título de la revista
Animals
Número de la revista
7
Datos de la obra
Caamaño, J. N., Tamargo, C., Parrilla, I., Martínez-Pastor, F., Padilla, L., Salman, A., Fueyo, C., Fernández, Á., Merino, M. J., Iglesias, T. and Hidalgo, C. O. (2021). Post-thaw sperm quality and functionality in the autochthonous pig breed Gochu Asturcelta. Animals (Basel), 11(7), Article e1885-. https://doi.org/10.3390/ani11071885
Editor
MDPI
Fecha
2021
Resumo
[EN] Genetic resource banks (GRB) preserve the genetic material of endangered, valuable indi-viduals or genetically relevant breeds. Semen cryopreservation is a crucial technique to reach these goals. Thus, we aimed to assess the sperm parameters of semen doses from the native pig breed Gochu Asturcelta stored at the GRB of Principado de Asturias (GRB-PA, Gijón, Spain), focusing on intrinsic and extrinsic (boar, season) factors. Two straws per boar (n = 18, 8–71 months of age) were thawed, pooled, and assessed after 30 and 150 min at 37 °C by CASA (computer-assisted sperm analysis system; motility and kinematic parameters) and flow cytometry (viability, acrosomal status, mitochondrial activity, apoptosis, reactive oxygen species, and chromatin status). The effects of age, incubation, and season on post-thawing quality were determined using linear mixed-effects models. Parameters were on the range for commercial boar breeds, with chromatin status (SCSA: fragmentation and immaturity) being excellent. Incubation decreased sperm quality and functionality. The boar age did not have a significant effect (p > 0.05), but the between-boar variability was significant (p < 0.001). The season significantly affected many parameters (motility, kinematics, viability, acro-somal status, mitochondrial activity), especially after 150 min of incubation. In general, samples collected in spring and summer showed higher quality post-thawing, the lowest in winter. In con-clusion, the sperm doses from the Gochu Asturcelta breed stored at the GRB-PA showed excellent chromatin status and acceptable characteristics after thawing. Therefore, boar and seasonal variability in this autochthonous breed could be relevant for cryobank management
Materia
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Peer review
SI
ID proyecto
- info:eu-repo/grantAgreement/INIA//RZP2017-00008-00-00/Acciones para la Conservación y Caracterización del Banco de Recursos Zoogenéticos de Razas Domésticas Autóctonas en Peligro de Desaparición del Principado de Asturias
- info:eu-repo/grantAgreement/AEI/Programa Estatal de I+D+i Orientada a los Retos de la Sociedad/RTI2018-095183-B-I00/ES/Incremento de la eficiencia reproductiva de la cría porcina mediante la aplicación de técnicas avanzadas de análisis de la cromatina espermática y selección por coloide
- info:eu-repo/grantAgreement/Junta de Castilla y León/Programa de apoyo a proyectos de investigación, cofinanciados por el Fondo Europeo de Desarrollo Regional/LE023P20/Mejora de la eficiencia de la industria porcina mediante la aplicación de técnicas avanzadas de selección espermática y análisis de la cromatina espermática
- info:eu-repo/grantAgreement/MECD/Ayudas a los Grupos y Unidades de Excelencia Científica de la Región de Murcia/SENECAFOUNDATION19892/GERM/15/Reproductive biotechnologies for livestock and human health
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