Título
Analysis of DNA damage after human sperm cryopreservation in genes crucial for fertilization and early embryo development
Autor
Facultad/Centro
Área de conocimiento
Título de la revista
Andrology
Número de la revista
5
Datos de la obra
Valcarce, Cartón-García, Riesco, Herráez, & Robles. (2013). Analysis of DNA damage after human sperm cryopreservation in genes crucial for fertilization and early embryo development. Andrology, 1(5), 723-730. https://doi.org/10.1111/J.2047-2927.2013.00116.X
Editor
Wiley
Fecha
2013
ISSN
2047-2919
Resumen
[EN] Sperm cryopreservation is widely used in clinic for insemination, in vitro fertilization and other procedures such as intracytoplasmic sperm injection. The assessment after freezing/thawing of spermatozoa viability, motility and sometimes DNA integrity (mainly using fragmentation assays) has been considered enough to guarantee the safety and effectiveness of the technique. However, it is known that, even when fragmentation is absent, a significant DNA damage could be detected in some genome regions. This is particularly important considering that, during the last years, several studies have pointed out the importance of key paternal genes in early embryo development. In this study, using normozoospermic donors, we present a candidate gene approach in which we quantify the number of lesions produced by freezing/thawing over key genes (PRM1, BIK, FSHB, PEG1/MEST, ADD1, ARNT, UBE3A, SNORD116/PWSAS) using quantitative PCR. Our results demonstrated that the cryopreservation protocol used, which is routinely employed in clinic, produced DNA lesions. The genes studied are differentially affected by the process, and genome regions related to Prader-Willi and Angelman syndromes were among the most damaged: SNORD116/PWSAS (4.56 ± 1.84 lesions/10 kb) and UBE3A (2.22 ± 1.3 lesions/10 kb). To check if vitrification protocols could reduce these lesions, another experiment was carried out studying some of those genes with higher differences in the first study (FSHB, ADD1, ARNT and SNORD116/PWSAS). The number of lesions was not significantly reduced compared to cryopreservation. These results could be relevant for the selection of the most adequate available cryopreservation protocol in terms of the number of lesions that produced over key genes, when no differences with other traditional techniques for DNA assessment could be detected.
Materia
Palabras clave
Idioma
eng
Tipo documental
info:eu-repo/semantics/article
Peer review
SI
URI
DOI
Versión del editor
https://onlinelibrary.wiley.com/doi/10.1111/j.2047-2927.2013.00116.x
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