GENERAL INFORMATION
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1. Title of Dataset: 
Cold-Shock Test Is a Practical Method for Selecting Boar Ejaculates Yielding Appropriate Seminal Plasma for Post-Thawing Supplementation


2. Authorship: 

Name: Martínez Pastor, Felipe
Institution: Universidad de León 
Email: felipe.martinez@unileon.es
ORCID: https://orcid.org/0000-0003-2987-4302


DESCRIPTION
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1. Dataset language: 
English 

2. Abstract:
Artificial insemination (AI) with cryopreserved semen is still unreliable for extensive pig indus-try application. 
Adding seminal plasma (SP) could improve post-thawing quality, but its suita-bility could vary. We applied a 
simple cold-shock test (CST, 5 min at 0 °C) on neat semen for clas-sifying ejaculates (n = 63) as resistant or sensitive, 
obtaining two SP pools (CST-resistant: SPr, sensitive: SPs). Subsequently, frozen/thawed spermatozoa from six boars were 
incubated (37 °C) in MR-A® extender (control), 20% SPr, or 20% SPs, and analyzed at 0, 2, and 4 h. SP improved total and progressive 
motility, with a higher effect for SPr and STR (p < 0.05), decreasing kine-matic parameters VCL and VAP, ALH, and BCF. 
Sperm viability was unaffected. SP increased apoptotic and membrane disorder ratios, and acrosomal damage, not affecting 
the chromatin structure (DNA fragmentation and immaturity by SCSA), protamination (CMA3), or disulfide levels (mBBr). 
However, the proportion of spermatozoa with elevated free thiols (disulfide bridges reduction) significantly increased.
 Results support a stimulatory role of SP on thawed semen, with additional benefits from SPr. The effect of SP and especially 
 SPr after AI should be tested since CST could be a practical test for selecting suitable ejaculates in AI centers. 
 This research was funded by the Ministry of Science and Innovation (Spain), grant number RTI2018-095183-B-I00 (MCI/AEI/FEDER, UE) 
 and LE023P20, Junta de Castilla y Le-ón/Consejería de Educación/FEDER, EU.

3. Keywords: 
Boar Seminal plasma Cryopreserved semen Spermatozoa Sperm chromatin

4. Date of data collection:
 2021

5. Publication Date:
 2021-03-15

6. Grant information:  

7. Geographical location/s of data collection:



ACCESS INFORMATION
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1. Dataset Creative Commons License:
CC BY-NC 4.0

2. Dataset DOI: 

3. Related publication:


METHODOLOGICAL INFORMATION
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1. Description of methods used for collection-generation of data: 


FILE OVERVIEW
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1. File List: 
 
2. Relationship between files:
 only one file

3. File format:
 RData


DATA-SPECIFIC INFORMATION
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