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dc.contributorFacultad de Ciencias Biologicas y Ambientaleses_ES
dc.contributor.authorMartínez Pastor, Felipe 
dc.contributor.authorFernández Santos, María Rocío
dc.contributor.authorDomínguez Rebolledo, Álvaro Efrén
dc.contributor.authorEsteso, Milagros
dc.contributor.authorGarde López-Brea, Julián
dc.contributor.otherBiologia Celulares_ES
dc.date2009-06
dc.date.accessioned2019-04-16T22:44:17Z
dc.date.available2019-04-16T22:44:17Z
dc.date.issued2019-04-17
dc.identifier.citationReproduction in Domestic Animals, 2009, vol. 44, n. 3es_ES
dc.identifier.otherhttps://onlinelibrary.wiley.com/doi/full/10.1111/j.1439-0531.2008.01098.xes_ES
dc.identifier.urihttp://hdl.handle.net/10612/10291
dc.descriptionP. 424-431es_ES
dc.description.abstractThe assessment of sperm chromatin status is compulsory in a complete spermiogram. Here we applied the sperm chromatin structure assay (SCSA) and the sperm chromatin dispersion (SCD) test to assess the chromatin status of three fighting bulls. Cryopreserved semen (two straws/bull) were analysed by duplicate after thawing and after 6 h at 37°C with and without oxidative stress (1 mm FE2+). Results (SCD: percentage of spermatozoa with halo; SCSA: SD‐DFI, %DFI and HDS) were analysed for differences between bulls and treatments, sensitivity and specificity (receiver operating characteristic curves) and repeatability (repeatability coefficients as 2SD of duplicate differences).%DFI for the three bulls was below 2% at 0 h, indicating no risk for fertility according to previous reports. It increased slightly for two of the bulls after FE2+ treatment (%DFI < 5%) and more pronouncedly for the other bull (C, %DFI∼10%), which merits further investigation. SCD rendered higher percentage of halos for bull C, but could not discriminate between samples with and without oxidizing treatment (AUC: 0.52). SCSA (%DFI) showed a high discriminating ability between treatments (AUC: 0.96). The repeatability coefficient was also higher for SCD (5.9) than for %DFI (1.8), indicating lower repeatability for SCD. Overall, %DFI might be the most useful parameter for assessing sperm chromatin on fighting bull. SCD might yield different information than SCSA, hence further research is warranted.es_ES
dc.languageenges_ES
dc.publisherJohn Wiley & Sonses_ES
dc.subjectVeterinariaes_ES
dc.subject.otherToros de lidiaes_ES
dc.subject.otherSemenes_ES
dc.subject.otherCromatinaes_ES
dc.subject.otherEstrés oxidativoes_ES
dc.titleDNA Status on Thawed Semen from Fighting Bull: A Comparison Between the SCD and the SCSA Testses_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.description.peerreviewedSIes_ES


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