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dc.contributorFacultad de Ciencias Biologicas y Ambientaleses_ES
dc.contributor.authorÁlvarez García, Mercedes 
dc.contributor.authorGarcía Macías, Vanesa
dc.contributor.authorMartínez Pastor, Felipe 
dc.contributor.authorMartínez, Félix
dc.contributor.authorBarragán Santos, Santiago
dc.contributor.authorMata Campuzano, María
dc.contributor.authorGarde López-Brea, Julián
dc.contributor.authorAnel Rodríguez, Luis 
dc.contributor.authorPaz Cabello, Paulino de 
dc.contributor.otherBiologia Celulares_ES
dc.date2008-12
dc.date.accessioned2019-04-16T23:03:58Z
dc.date.available2019-04-16T23:03:58Z
dc.date.issued2019-04-17
dc.identifier.citationTheriogenology, 2008, vol. 70, n. 9es_ES
dc.identifier.otherhttps://www.sciencedirect.com/science/article/pii/S0093691X08004652#!es_ES
dc.identifier.urihttp://hdl.handle.net/10612/10317
dc.descriptionP. 1498-1506es_ES
dc.description.abstractThe Cantabrian brown bear (Ursus arctos) is a highly endangered species in Spain and basic studies are necessary in order to bank its germplasm. Sperm heads are mainly made up of chromatin, thus their shape depends partly on chromatin structure. Thawed semen from 10 bears was used to analyze chromatin status by sperm chromatin structure assay (SCSA) and head morphometry by the computer-assisted sperm morphology assessment (CASMA) system. Morphometry was analyzed before and after freezing–thawing in order to evaluate the effects of cryopreservation on sperm heads. Each spermatozoon was measured for four primary parameters (length, L; width, W; area, A; perimeter, P) and derived parameters (ellipticity: L/W, circularity: 4πA/P2, elongation: (L − W)/(L + W), regularity: πLW/4A). All the derived parameters significantly differed between bears. Likewise, cryopreservation affected head morphometry by reducing its size. Clustering based on morphometric parameters separated three subpopulations, one of them being significantly more influenced by the cryopreservation process. We obtained high correlations between head morphometry and SCSA parameters: standard deviation of DNA fragmentation index (SD-DFI) was correlated with perimeter and area (r = 0.75 and r = 0.62, respectively) and DFIm and DFIt (moderate and total DNA fragmentation index) were correlated with perimeter (r = 0.65 and r = 0.67, respectively). Nevertheless, classification of males according to SCSA or head morphometry did not completely agree so the two assays might explain male variability differently. We conclude that cryopreservation affected morphometry at least in a subset of spermatozoa. These results might improve future application of sperm banking techniques in this species.es_ES
dc.languageenges_ES
dc.publisherElsevieres_ES
dc.subjectVeterinariaes_ES
dc.subject.otherBeares_ES
dc.subject.otherSpermes_ES
dc.subject.otherCryopreservationes_ES
dc.subject.otherMorphometryes_ES
dc.subject.otherChromatines_ES
dc.titleEffects of cryopreservation on head morphometry and its relation with chromatin status in brown bear (Ursus arctos) spermatozoaes_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.description.peerreviewedSIes_ES


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