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    Título
    A pilot study on post-thawing quality of Iberian red deer spermatozoa (epididymal and electroejaculated) depending on glycerol concentration and extender osmolality
    Autor
    Martínez Pastor, FelipeAutoridad BuleriaORCID
    Martínez, Félix
    García Macías, Vanesa
    Esteso, Milagros
    Anel Rodríguez, Enrique
    Fernández Santos, María Rocío
    Soler, Ana J.
    Paz Cabello, Paulino deAutoridad BuleriaORCID
    Garde López-Brea, Julián
    Anel Rodríguez, LuisAutoridad BuleriaORCID
    Facultad/Centro
    Facultad de Ciencias Biologicas y Ambientales
    Área de conocimiento
    Biologia Celular
    Datos de la obra
    Theriogenology, 2006, vol. 66, n. 5
    Editor
    Elsevier
    Fecha
    2006-09-15
    Abstract
    The optimization of cryopreservation extenders is a fundamental issue for adequately performing germplasm banking on wild species. We have tested two glycerol concentrations (4 and 8%), and three extender osmolalities (320, 380 and 430 mOsm/kg; before adding cryoprotectants), for cryopreservation of epididymal and ejaculated sperm samples from Iberian red deer. All the extenders were based on Tes–Tris and fructose (for osmolality adjustment), and complemented with 20% egg yolk. Epididymal and ejaculated sperm samples were obtained from the cauda epididymis (post-mortem) and using electroejaculation, respectively. Samples were diluted 1:1 with each extender and equilibrated for 2 h at 5 °C. Then, they were diluted down to 100 × 106 sperm/mL and frozen at −20 °C/min. Post-thawed samples were assessed for motility (CASA), HOS test, proportion of swollen (osmotically challenged) cells in the untreated sample, viability and acrosomal status. For epididymal samples, 8% glycerol rendered a slightly higher proportion of intact acrosomes on viable spermatozoa than 4%; regarding extender osmolality, 380 and 430 mOsm/kg rendered higher motility results, and the 430 mOsm/kg yielded the lowest proportion of swollen spermatozoa. For ejaculated samples, 4% glycerol yielded more viable spermatozoa than 8%; for extender osmolality, 320 mOsm/kg rendered the highest percentages of progressively motile and viable spermatozoa, although 380 mOsm/kg extender was not significantly different. These results show that sample source influences extender suitability, and that extenders should be isoosmotic or rather slightly hyperosmotic. Future studies should test multiple glycerol concentrations and extender osmolalities in order to adjust them to these kinds of sample.
    Materia
    Veterinaria
    Palabras clave
    Sperm cryopreservation
    Epididymis
    Electroejaculation
    Extender
    Red deer
    Peer review
    SI
    URI
    http://hdl.handle.net/10612/10337
    Versión del editor
    https://www.sciencedirect.com/science/article/pii/S0093691X06001993#!
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