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    Título
    Undiluted or extended storage of ram epididymal spermatozoa as alternatives to refrigerating the whole epididymes
    Autor
    Tamayo Canul, Julio Renan
    Álvarez García, MercedesAutoridad BuleriaORCID
    López Urueña, Elena
    Nicolás, M.
    Martínez Pastor, FelipeAutoridad BuleriaORCID
    Anel Rodríguez, Enrique
    Anel Rodríguez, LuisAutoridad BuleriaORCID
    Paz Cabello, Paulino deAutoridad BuleriaORCID
    Facultad/Centro
    Facultad de Ciencias Biologicas y Ambientales
    Área de conocimiento
    Biologia Celular
    Datos de la obra
    Animal Reproduction Science, 2011 vol. 126, n. 1–2
    Editor
    Elsevier
    Fecha
    2011-06
    Abstract
    The effect of storage procedure at 5 °C on the quality of ram spermatozoa from the cauda epididymis was analyzed. Two strategies were tested at 0, 24, 48 and 72 h post-mortem: (1) spermatozoa held in the epididymal fluid and stored either in the cauda epididymis (In-EPID) or in vitro (Ex-EPID), (2) epididymal spermatozoa extended in three media at 320, 370 and 420 mOsm/kg (D320, D370, D420). Analyzed parameters were: osmolality, pH, motility, acrosomal status and viability. In experiment 1, osmolality of the In-EPID samples, but not in Ex-EPID, increased with post-mortem time. Motility of In-EPID spermatozoa in samples, after 24 h post-mortem, was higher compared to the Ex-EPID samples, although differences decreased at 48 and 72 h. In experiment 2, total (TM) and progressive motility (PM) were not significantly affected by storage time for D320 and In-EPID samples. However, the motility of D370 and D420 samples significantly decreased with time. TM and PM of D320 were significantly higher than D370 and D420 at 72 h. At 24 h, sperm viability was higher for In-EPID (80.7 ± 3.4%) than for the extended samples (44.8 ± 2.9%, 37.7 ± 3.9% and 48.6 ± 6.0% for D320, D370 and D420, respectively), which also decreased faster with time. At 24 h, the percentage of damaged acrosomes was low and similar for the four methods of storage, but damaged acrosomes increased with time for D320 and D370. Storing the spermatozoa in the epididymis is a good strategy for maintaining sperm quality in ram, at least for 48 h. The D320 extender preserve motility of epididymal spermatozoa but does not protect the status of the acrosome.
    Materia
    Veterinaria
    Palabras clave
    Ram
    Epididymis
    Refrigerated storage
    Osmolality
    Extender
    Sperm quality
    Peer review
    SI
    URI
    http://hdl.handle.net/10612/10533
    Versión del editor
    https://www.sciencedirect.com/science/article/pii/S0378432011001175#!
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