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dc.contributorFacultad de Ciencias Biologicas y Ambientaleses_ES
dc.contributor.authorDomínguez Rebolledo, Álvaro Efrén
dc.contributor.authorMartínez Pastor, Felipe 
dc.contributor.authorBisbal Vigo, Alfonso
dc.contributor.authorRos Santaella, José Luis
dc.contributor.authorGarcía Álvarez, Olga
dc.contributor.authorMaroto Morales, Alejandro
dc.contributor.authorSoler, Ana J.
dc.contributor.authorGarde López-Brea, Julián
dc.contributor.authorFernández Santos, María Rocío
dc.contributor.otherBiologia Celulares_ES
dc.date2011-06
dc.date.accessioned2019-04-23T00:12:16Z
dc.date.available2019-04-23T00:12:16Z
dc.date.issued2019-04-23
dc.identifier.citationReproduction in Domestic Animals, 2011, vol. 46, n. 3es_ES
dc.identifier.otherhttps://onlinelibrary.wiley.com/doi/full/10.1111/j.1439-0531.2010.01677.xes_ES
dc.identifier.urihttp://hdl.handle.net/10612/10612
dc.descriptionP. 393-403es_ES
dc.description.abstractOxidative stress represents a challenge during sperm manipulation. We have tested the effect of increasing hydrogen peroxide (H2O2) levels on red deer spermatozoa after cryopreservation, and the role of male‐to‐male variation in that response. In a first experiment, eight thawed samples were submitted to 0, 25, 50, 100 and 200 μm H2O2 for 2 h at 37°C. Intracellular reactive oxygen species (H2DCFDA‐CM) increased with H2O2 concentration, but we only detected a decrease in sperm function (motility by CASA and chromatin damage by sperm chromatin structure assay) with 200 μm. Lipoperoxidation assessed by the thiobarbituric acid reactive substance (TBARS) method increased slightly with 50 μm H2O2 and above. In a second experiment, samples from seven males were submitted to 0 and 200 μm H2O2 for 2 h, triplicating the experiment within each male. Males differed at thawing and regarding their response to incubation and H2O2 presence. We found that the kinematic parameters reflected male‐to‐male variability, whereas the response of the different males was similar for lipid peroxidation and viability. A multiparametric analysis showed that males grouped differently if samples were assessed after thawing, after incubation without H2O2 or after incubation with H2O2. Red deer spermatozoa are relatively resilient to H2O2 after thawing, but it seems to be a great male‐to‐male variability regarding the response to oxidative stress. The acknowledgement of this individual variability might improve the development of optimized sperm work protocols.es_ES
dc.languageenges_ES
dc.publisherJohn Wiley & Sonses_ES
dc.subjectVeterinariaes_ES
dc.subject.otherCiervo rojoes_ES
dc.subject.otherEspermaes_ES
dc.subject.otherAguaes_ES
dc.subject.otherEstrés oxidativoes_ES
dc.titleResponse of Thawed Epidi dymal Red Deer Spermatozoa to Increasing Concentrations of Hydrogen Peroxide, and Importance of Individual Male Variabilityes_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.description.peerreviewedSIes_ES


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