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dc.contributorFacultad de Ciencias Biologicas y Ambientaleses_ES
dc.contributor.authorMata Campuzano, María
dc.contributor.authorÁlvarez Rodríguez, Manuel
dc.contributor.authorÁlvarez García, Mercedes
dc.contributor.authorAnel Rodríguez, Luis 
dc.contributor.authorPaz Cabello, Paulino de
dc.contributor.authorGarde López-Brea, Julián
dc.contributor.authorMartínez Pastor, Felipe 
dc.contributor.otherBiologia Celulares_ES
dc.date2012-12
dc.date.accessioned2019-04-25T13:48:46Z
dc.date.available2019-04-25T13:48:46Z
dc.date.issued2019-04-25
dc.identifier.citationReproduction in Domestic Animals, 2012, vol. 47, n. 6es_ES
dc.identifier.otherhttps://onlinelibrary.wiley.com/doi/10.1111/j.1439-0531.2012.01990.xes_ES
dc.identifier.urihttp://hdl.handle.net/10612/10656
dc.descriptionP. 907-914es_ES
dc.description.abstractThawed ram spermatozoa were incubated at 37°C in the presence of dehydroascorbic acid (DHA), TEMPOL (TPL), N‐acetyl‐cysteine (NAC) and rutin (RUT), at 0.1 and 1 mm, in order to test their effects on sperm physiology. Cryopreserved spermatozoa from four rams were thawed, pooled, washed and incubated in TALP‐Hepes with 1 mm or 0.1 mm of each antioxidant, performing a replicate with induced oxidative stress (Fe2+/ascorbate). Motility (CASA), viability and mitochondrial membrane potential (flow cytometry) were analysed at 2 and 4 h. Lipoperoxidation (MDA production), intracellular reactive oxygen species (ROS) and DNA status (TUNEL) were analysed at 4 h. Antioxidants, except DHA 0.1 mm, decreased motility and kinematic parameters, but had little effect on viability or mitochondrial activity. Except 1 mm DHA, the antioxidants reduced ROS at 4 h. Moreover, NAC 1 mm, rutin and TEMPOL reduced ROS and DNA damage in the presence of oxidative stress. N‐acetyl‐cysteine, rutin 1 mm and TEMPOL reduced lipoperoxidation in the presence of oxidative stress. However, DHA did not affect lipoperoxidation. At 1 mm, DHA increased DNA damage in the absence of oxidative stress. Dehydroascorbic acid effects could arise from spermatozoa having a low capacity for reducing it to ascorbic acid, and it may be tested in the presence of other antioxidants or reducing power. Future research should focus in testing whether the inhibition of motility observed for NAC, rutin and TEMPOL is reversible. These antioxidants might be useful at lower temperatures (refrigerated storage or cryopreservation) when their protective effects could be advantageous.es_ES
dc.languageenges_ES
dc.publisherJohn Wiley & Sonses_ES
dc.subjectVeterinariaes_ES
dc.subject.otherAntioxidanteses_ES
dc.subject.otherEstrés oxidativoes_ES
dc.subject.otherGanado ovinoes_ES
dc.subject.otherEspermaes_ES
dc.titleEffect of Several Antioxidants on Thawed Ram Spermatozoa Submitted to 37°C up to Four Hourses_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.description.peerreviewedSIes_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES


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