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    Título
    Reduced glutathione and Trolox (vitamin E) as extender supplements in cryopreservation of red deer epididymal spermatozoa
    Autor
    Anel López, LuisAutoridad Buleria ORCID
    Álvarez Rodríguez, Manuel
    García Álvarez, Olga
    Álvarez García, MercedesAutoridad Buleria ORCID
    Maroto Morales, Alejandro
    Anel Rodríguez, LuisAutoridad Buleria ORCID
    Paz Cabello, Paulino deAutoridad Buleria ORCID
    Garde López-Brea, Julián
    Martínez Pastor, FelipeAutoridad Buleria ORCID
    Facultad/Centro
    Facultad de Ciencias Biologicas y Ambientales
    Área de conocimiento
    Biologia Celular
    Datos de la obra
    Animal Reproduction Science, 2012, vol. 135, n. 1–4
    Editor
    Elsevier
    Fecha
    2012-11
    Descripción física
    P. 37-46
    Abstract
    The use of assisted reproductive techniques in cervids is increasing as the commercial use of these species increase. We have tested the suitability of the antioxidants Trolox and reduced glutathione (GSH) for freezing red deer epididymal spermatozoa, aiming at improving post-thawing quality. Samples from 19 stags were frozen in a TES-Tris-fructose extender (20% egg yolk, 8% glycerol), with 1 or 5 mM of antioxidant. Motility (CASA), lipoperoxidation (malondialdehyde –MDA– production), membrane status, mitochondrial activity, acrosomal status (flow cytometry) and chromatin status (SCSA: %DFI and %HDS; flow cytometry) were assessed after thawing and after 6 h at 39 °C. There were few differences between treatments after thawing, with Trolox reducing MDA production in a dose–response manner. After the incubation, sperm quality decreased and %DFI increased moderately, with no change for MDA. GSH improved motility, kinematic parameters and mitochondrial status, with a slight increase in %HDS. GSH 5 mM also increased moderately MDA production and %DFI, possibly due to enhanced metabolic activity and reducing power. Trolox maintained MDA low, but was detrimental to sperm quality. Trolox might not be appropriate for the cryopreservation of red deer epididymal spermatozoa, at least at the millimolar range. GSH results are promising, especially regarding motility improvement after the post-thawing incubation, and should be selected for future fertility trials.
    Materia
    Veterinaria
    Palabras clave
    Epididymal sperm
    Red deer
    Antioxidant
    Trolox
    GSH
    Cryopreservation
    Idioma
    eng
    Tipo documental
    info:eu-repo/semantics/article
    Peer review
    SI
    URI
    http://hdl.handle.net/10612/10658
    Versión del editor
    https://www.sciencedirect.com/science/article/pii/S0378432012002758?via%3Dihub
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    • Artículos de revista (post-print) [2091]
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