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    Título
    The percentage of spermatozoa lost during the centrifugation of brown bear (Ursus arctos) ejaculates is associated with some spermatozoa quality and seminal plasma characteristics
    Autor
    Álvarez García, MercedesAutoridad BuleriaORCID
    Nicolás, M.
    Barragán Santos, Santiago
    López Urueña, Elena
    Anel López, LuisAutoridad BuleriaORCID
    Martínez Pastor, FelipeAutoridad BuleriaORCID
    Tamayo Canul, Julio Renan
    Anel Rodríguez, LuisAutoridad BuleriaORCID
    Paz Cabello, Paulino deAutoridad BuleriaORCID
    Facultad/Centro
    Facultad de Ciencias Biologicas y Ambientales
    Área de conocimiento
    Biologia Celular
    Título de la revista
    Animal Reproduction Science
    Datos de la obra
    Animal Reproduction Science, 2012, vol. 135, n. 1–4
    Editor
    Elsevier
    Fecha
    2012-11
    Abstract
    Cryopreservation of brown bear (Ursus arctos) semen requires centrifugation to increase concentration and/or remove urine contamination. However, a percentage of the spermatozoa are lost in the process. This percentage varies considerably between males and ejaculates, and we have studied the effect of sperm quality and seminal plasma characteristics on the spermatozoa recovery rate after centrifugation. One hundred and thirty one sperm samples obtained from fifteen brown bear males by electroejaculation under general anaesthesia were used. The ejaculates were centrifuged 600 × g for 6 min. Motility was assessed by CASA, and acrosomal status (PNA-FITC) and viability (SYBR-14/propidium iodide) were determined by flow cytometry. Seminal plasma characteristics (albumin, alkaline phosphatase, calcium, cholesterol, creatine, glucose, glutamic oxaloacetic transaminase (GOT), lactate, lipase, magnesium, phosphate and total protein) were determined by a biochemical and gas analysis. Total motility (r = 0.26; P = 0.005) and cell viability (r = 0.20; P = 0.033) were positively correlated with the percentage of recovered spermatozoa. Sperm recovery was correlated with the concentration of several components of seminal plasma: negatively with glucose concentration (r = −0.47; P = 0.005) and positively with the enzymes GOT (r = 0.36; P = 0.040) and lactate dehydrogenase (r = 0.36; P = 0.041). After sorting the data into classes according to sperm recovery (Low: 0–39, Medium: 40–69, High: 70–100), we observed that the samples with a lower recovery rate derived from ejaculates with lower values for TM, VAP and viability (P < 0.05). Multiple regression analysis rendered two models to define the post-centrifugation spermatozoa recovery which included total motility and damaged acrosome or glucose, GOT and lactate dehydrogenase. We discuss these relationships and their implications in the electroejaculation procedure and the handling of the sample during centrifugation.
    Materia
    Veterinaria
    Palabras clave
    Centrifugation
    Brown bear
    Semen
    Seminal plasma
    Sperm recovery
    Peer review
    SI
    URI
    http://hdl.handle.net/10612/10659
    Versión del editor
    https://www.sciencedirect.com/science/article/pii/S0378432012002990#!
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