Mostrar el registro sencillo del ítem

dc.contributorFacultad de Ciencias Biologicas y Ambientaleses_ES
dc.contributor.authorPaz Cabello, Paulino de 
dc.contributor.authorÁlvarez Rodríguez, Manuel
dc.contributor.authorNicolás, M.
dc.contributor.authorÁlvarez García, Mercedes 
dc.contributor.authorChamorro Álvarez, César Ángel 
dc.contributor.authorBarragán Santos, Santiago
dc.contributor.authorMartínez Pastor, Felipe 
dc.contributor.authorAnel Rodríguez, Luis 
dc.contributor.otherBiologia Celulares_ES
dc.date2012-02
dc.date.accessioned2019-04-25T13:50:57Z
dc.date.available2019-04-25T13:50:57Z
dc.date.issued2019-04-25
dc.identifier.citationReproduction in Domestic Animals, 2012, vol. 47, n. 1es_ES
dc.identifier.otherhttps://onlinelibrary.wiley.com/doi/full/10.1111/j.1439-0531.2011.01808.xes_ES
dc.identifier.urihttp://hdl.handle.net/10612/10663
dc.descriptionP. 105-112es_ES
dc.description.abstractIn order to establish a semen bank for the endangered Cantabrian brown bear, we tested five glycerol concentrations and three freezing rates for electroejaculated semen. Electroejaculation was performed on nine males. Semen was diluted in TES–Tris–Fructose (20% egg yolk, 2% EDTA, 1% Equex) with 2%, 4%, 6%, 8% or 10% glycerol and frozen at −10, −20 or −40°C/min. Before and after cryopreservation, samples were analysed for motility (CASA), viability and acrosomal status (flow cytometry). Pre‐freezing results showed that glycerol concentration had no significant effect on total motility or progressive motility, but it significantly decreased VCL, ALH, viability and acrosomal status (p < 0.05). After thawing, sperm motility was higher at extender with 4%, 6% and 8% glycerol, but only at 4% and 6% glycerol for viability and acrosomal status. For 4% and 6% glycerol, freezing rates did not have significant effects. The curve fitting gave an estimate of the optimal glycerol concentration, with all the optimal values for every parameter between 6% and 7% glycerol falling. We propose using 6% glycerol and a freezing velocity of −20°C/min for freezing brown bear ejaculated spermatozoa.es_ES
dc.languageenges_ES
dc.publisherJohn Wiley & Sonses_ES
dc.subjectVeterinariaes_ES
dc.subject.otherGlicerinaes_ES
dc.subject.otherCriopreservaciónes_ES
dc.subject.otherEspermaes_ES
dc.subject.otherOsos pardoses_ES
dc.subject.otherUrsus arctoses_ES
dc.titleOptimization of Glycerol Concentration and Freezing Rate in the Cryopreservation of Ejaculate From Brown Bear (Ursus arctos)es_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.description.peerreviewedSIes_ES


Ficheros en el ítem

Thumbnail

Este ítem aparece en la(s) siguiente(s) colección(ones)

Mostrar el registro sencillo del ítem