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dc.contributorFacultad de Ciencias Biologicas y Ambientaleses_ES
dc.contributor.authorFernández Gago, Rocío
dc.contributor.authorDomínguez Fernández De Tejerina, Juan Carlos 
dc.contributor.authorMartínez Pastor, Felipe 
dc.contributor.otherBiologia Celulares_ES
dc.date2013-09-01
dc.date.accessioned2019-05-07T09:31:50Z
dc.date.available2019-05-07T09:31:50Z
dc.date.issued2019-05-07
dc.identifier.citationTheriogenology, 2013, vol. 80, n. 4es_ES
dc.identifier.otherhttps://www.sciencedirect.com/science/article/pii/S0093691X13001854#!es_ES
dc.identifier.urihttp://hdl.handle.net/10612/10668
dc.descriptionP. 400-410es_ES
dc.description.abstractCryopreservation induces extensive biophysical and biochemical changes in the sperm. In the present study, we used flow cytometry to assess the capacitation-like status of frozen-thawed boar spermatozoa and its relationship with intracellular calcium, assessment of membrane fluidity, modification of thiol groups in plasma membrane proteins, reactive oxygen species (ROS) levels, viability, acrosomal status, and mitochondrial activity. This experiment was performed to verify the effect of adding seminal plasma on post-thaw sperm functions. To determine these effects after cryopreservation, frozen-thawed semen from seven boars was examined after supplementation with different concentrations of pooled seminal plasma (0%, 10%, and 50%) at various times of incubation from 0 to 4 hours. Incubation caused a decrease in membrane integrity and an increase in acrosomal damage, with small changes in other parameters (P > 0.05). Although 10% seminal plasma showed few differences with 0% (ROS increase at 4 hours, P < 0.05), 50% seminal plasma caused important changes. Membrane fluidity increased considerably from the beginning of the experiment, and ROS and free thiols in the cell surface increased by 2 hours of incubation. By the end of the experiment, viability decreased and acrosomal damage increased in the 50% seminal plasma samples. The addition of 50% of seminal plasma seems to modify the physiology of thawed boar spermatozoa, possibly through membrane changes and ROS increase. Although some effects were detrimental, the stimulatory effect of 50% seminal plasma could favor the performance of post-thawed boar semen, as showed in the field (García JC, Domínguez JC, Peña FJ, Alegre B, Gonzalez R, Castro MJ, Habing GG, Kirkwood RN. Thawing boar semen in the presence of seminal plasma: effects on sperm quality and fertility. Anim Reprod Sci 2010;119:160–5).es_ES
dc.languageenges_ES
dc.publisherElsevieres_ES
dc.subjectVeterinariaes_ES
dc.subject.otherBoares_ES
dc.subject.otherSemenes_ES
dc.subject.otherSeminal plasmaes_ES
dc.subject.otherSperm physiologyes_ES
dc.subject.otherFlow cytometryes_ES
dc.titleSeminal plasma applied post-thawing affects boar sperm physiology: A flow cytometry studyes_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.description.peerreviewedSIes_ES


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