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    Título
    Evaluation of the qualitative and quantitative effectiveness of three media of centrifugation (Maxifreeze, Cushion Fluid Equine, and PureSperm 100) in preparation of fresh or frozen-thawed brown bear spermatozoa
    Autor
    Nicolás, M.
    Álvarez García, MercedesAutoridad BuleriaORCID
    Barragán Santos, Santiago
    Martínez Pastor, FelipeAutoridad BuleriaORCID
    Chamorro Álvarez, César ÁngelAutoridad BuleriaORCID
    Álvarez Rodríguez, Manuel
    Paz Cabello, Paulino deAutoridad BuleriaORCID
    Anel Rodríguez, LuisAutoridad BuleriaORCID
    Facultad/Centro
    Facultad de Ciencias Biologicas y Ambientales
    Área de conocimiento
    Biologia Celular
    Datos de la obra
    Theriogenology, 2012, vol. 77, n. 6
    Editor
    Elsevier
    Fecha
    2012-04-01
    Abstract
    Centrifugation is a crucial procedure in sperm cryopreservation protocols of brown bear (Ursus arctos), because the semen must be processed to increase sperm concentration and/or clean urine-contaminated samples. The efficacy of three media for centrifugation (Maxifreeze [IMV technologies, L'Aigle, France], Cushion Fluid Equine (Minitübe, Tiefenbach, Germany), and PureSperm [Nidacon, Gothenburg, Sweden]) on the quality of bear spermatozoa was evaluated. In experiment one, two cushioned media used for protecting against mechanical stress during centrifugation were analyzed. In experiment two, a density gradient based on PureSperm was assessed in relation to the maximum retrieval and the quality of fresh spermatozoa, and the freezability of the spermatozoa selected in this density gradient was studied in experiment three. Finally, the selection of frozen-thawed sperm using PureSperm was analyzed in experiment four. Our results indicate that the use of dense isotonic cushion solutions (Maxifreeze, Cushion Fluid Equine) in centrifugation did not improve the quality of recovered spermatozoa compared with standard centrifugation. However, a density gradient prepared with PureSperm improved the quality of spermatozoa in fresh semen and frozen-thawed semen, but the spermatozoa selected from the fresh sample with this density gradient did not show a better resistance to freezing with this density gradient in comparison with the control sample.
    Materia
    Veterinaria
    Palabras clave
    Centrifugation
    Density gradient
    Cryopreservation
    Brown bear
    Spermatozoa
    Peer review
    SI
    URI
    http://hdl.handle.net/10612/10673
    Versión del editor
    https://www.sciencedirect.com/science/article/pii/S0093691X11005310
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