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dc.contributorFacultad de Ciencias Biologicas y Ambientaleses_ES
dc.contributor.authorNicolás, María
dc.contributor.authorÁlvarez García, Mercedes
dc.contributor.authorBarragán, Santiago
dc.contributor.authorMartínez Pastor, Felipe 
dc.contributor.authorChamorro Álvarez, César Ángel 
dc.contributor.authorÁlvarez Rodríguez, Manuel
dc.contributor.authorPaz Cabello, Paulino de
dc.contributor.authorAnel Rodríguez, Luis 
dc.contributor.otherBiologia Celulares_ES
dc.date2012-04-01
dc.date.accessioned2019-05-07T10:11:58Z
dc.date.available2019-05-07T10:11:58Z
dc.date.issued2019-05-07
dc.identifier.citationTheriogenology, 2012, vol. 77, n. 6es_ES
dc.identifier.otherhttps://www.sciencedirect.com/science/article/pii/S0093691X11005310es_ES
dc.identifier.urihttp://hdl.handle.net/10612/10673
dc.descriptionP. 1119-1128es_ES
dc.description.abstractCentrifugation is a crucial procedure in sperm cryopreservation protocols of brown bear (Ursus arctos), because the semen must be processed to increase sperm concentration and/or clean urine-contaminated samples. The efficacy of three media for centrifugation (Maxifreeze [IMV technologies, L'Aigle, France], Cushion Fluid Equine (Minitübe, Tiefenbach, Germany), and PureSperm [Nidacon, Gothenburg, Sweden]) on the quality of bear spermatozoa was evaluated. In experiment one, two cushioned media used for protecting against mechanical stress during centrifugation were analyzed. In experiment two, a density gradient based on PureSperm was assessed in relation to the maximum retrieval and the quality of fresh spermatozoa, and the freezability of the spermatozoa selected in this density gradient was studied in experiment three. Finally, the selection of frozen-thawed sperm using PureSperm was analyzed in experiment four. Our results indicate that the use of dense isotonic cushion solutions (Maxifreeze, Cushion Fluid Equine) in centrifugation did not improve the quality of recovered spermatozoa compared with standard centrifugation. However, a density gradient prepared with PureSperm improved the quality of spermatozoa in fresh semen and frozen-thawed semen, but the spermatozoa selected from the fresh sample with this density gradient did not show a better resistance to freezing with this density gradient in comparison with the control sample.es_ES
dc.languageenges_ES
dc.publisherElsevieres_ES
dc.subjectVeterinariaes_ES
dc.subject.otherCentrifugationes_ES
dc.subject.otherDensity gradientes_ES
dc.subject.otherCryopreservationes_ES
dc.subject.otherBrown beares_ES
dc.subject.otherSpermatozoaes_ES
dc.titleEvaluation of the qualitative and quantitative effectiveness of three media of centrifugation (Maxifreeze, Cushion Fluid Equine, and PureSperm 100) in preparation of fresh or frozen-thawed brown bear spermatozoaes_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.description.peerreviewedSIes_ES


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