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    Título
    Sperm concentration at freezing affects post-thaw quality and fertility of ram semen
    Autor
    Álvarez García, MercedesAutoridad Buleria ORCID
    Tamayo Canul, Julio Renan
    Anel Rodríguez, Enrique
    Boixo, Juan C.
    Mata Campuzano, María
    Martínez Pastor, FelipeAutoridad Buleria ORCID
    Anel Rodríguez, LuisAutoridad Buleria ORCID
    Paz Cabello, Paulino deAutoridad Buleria ORCID
    Facultad/Centro
    Facultad de Ciencias Biologicas y Ambientales
    Área de conocimiento
    Biologia Celular
    Datos de la obra
    Theriogenology, 2012, vol. 77, n. 6
    Editor
    Elsevier
    Fecha
    2012-04-01
    Descripción física
    P. 1111-1118
    Abstract
    We have investigated the effect of sperm concentration in the freezing doses 200, 400, 800, and 1600 × 106 mL−1 on the post-thaw quality and fertility of ram semen. Semen was collected from seven adult Churra rams by artificial vagina during the breeding season. The semen was diluted in an extender (TES-Tris-fructose, 20% egg yolk, and 4% glycerol), to a final concentration of 200, 400, 800, or 1600 × 106 mL−1 and frozen. Doses were analyzed post-thawing for motility (computer-assisted sperm analysis system [CASA]), viability, and acrosomal status (fluorescence probes propidium iodide [PI]/peanut agglutinin conjugated with fluorescein thiocyanate (PNA-FITC), SYBR-14/PI [Invitrogen; Barcelona, Spain] and YO-PRO-1/PI [Invitrogen; Barcelona, Spain]). Total motility and velocity were lower for 1600 × 106 mL−1 doses, while progressive motility and viability were lower both for 800 and 1600 × 106 mL−1. The proportion of viable spermatozoa showing increased membrane permeability (YO-PRO-1+) rose in 800 and 1200 × 106 mL−1. Intrauterine inseminations were performed with the 200, 400, and 800 × 106 mL−1 doses at a fixed sperm number (25 × 106 per uterine horn) in synchronized ewes. Fertility (lambing rate) was similar for semen frozen at 200 (57.5%) or 400 × 106 mL−1 (54.4%), whereas it was significantly lower for 800 × 106 mL−1 (45.5%). In conclusion, increasing sperm concentration in cryopreserved semen, at least at 800 × 106 mL−1 and more, adversely affects the postthawing quality and fertility of ram semen.
    Materia
    Veterinaria
    Palabras clave
    Ram
    Sperm cryopreservation
    Sperm concentration
    Sperm quality
    Fertility
    Idioma
    eng
    Tipo documental
    info:eu-repo/semantics/article
    Peer review
    SI
    URI
    http://hdl.handle.net/10612/10675
    Versión del editor
    https://www.sciencedirect.com/science/article/pii/S0093691X11005280
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    • Artículos de revista (post-print) [2096]
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