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dc.contributor | Facultad de Ciencias Biologicas y Ambientales | es_ES |
dc.contributor.author | Álvarez García, Mercedes | |
dc.contributor.author | Tamayo Canul, Julio Renan | |
dc.contributor.author | Anel Rodríguez, Enrique | |
dc.contributor.author | Boixo, Juan C. | |
dc.contributor.author | Mata Campuzano, María | |
dc.contributor.author | Martínez Pastor, Felipe | |
dc.contributor.author | Anel Rodríguez, Luis | |
dc.contributor.author | Paz Cabello, Paulino de | |
dc.contributor.other | Biologia Celular | es_ES |
dc.date | 2012-04-01 | |
dc.date.accessioned | 2019-05-07T10:45:26Z | |
dc.date.available | 2019-05-07T10:45:26Z | |
dc.date.issued | 2019-05-07 | |
dc.identifier.citation | Theriogenology, 2012, vol. 77, n. 6 | es_ES |
dc.identifier.other | https://www.sciencedirect.com/science/article/pii/S0093691X11005280 | es_ES |
dc.identifier.uri | http://hdl.handle.net/10612/10675 | |
dc.description | P. 1111-1118 | es_ES |
dc.description.abstract | We have investigated the effect of sperm concentration in the freezing doses 200, 400, 800, and 1600 × 106 mL−1 on the post-thaw quality and fertility of ram semen. Semen was collected from seven adult Churra rams by artificial vagina during the breeding season. The semen was diluted in an extender (TES-Tris-fructose, 20% egg yolk, and 4% glycerol), to a final concentration of 200, 400, 800, or 1600 × 106 mL−1 and frozen. Doses were analyzed post-thawing for motility (computer-assisted sperm analysis system [CASA]), viability, and acrosomal status (fluorescence probes propidium iodide [PI]/peanut agglutinin conjugated with fluorescein thiocyanate (PNA-FITC), SYBR-14/PI [Invitrogen; Barcelona, Spain] and YO-PRO-1/PI [Invitrogen; Barcelona, Spain]). Total motility and velocity were lower for 1600 × 106 mL−1 doses, while progressive motility and viability were lower both for 800 and 1600 × 106 mL−1. The proportion of viable spermatozoa showing increased membrane permeability (YO-PRO-1+) rose in 800 and 1200 × 106 mL−1. Intrauterine inseminations were performed with the 200, 400, and 800 × 106 mL−1 doses at a fixed sperm number (25 × 106 per uterine horn) in synchronized ewes. Fertility (lambing rate) was similar for semen frozen at 200 (57.5%) or 400 × 106 mL−1 (54.4%), whereas it was significantly lower for 800 × 106 mL−1 (45.5%). In conclusion, increasing sperm concentration in cryopreserved semen, at least at 800 × 106 mL−1 and more, adversely affects the postthawing quality and fertility of ram semen. | es_ES |
dc.language | eng | es_ES |
dc.publisher | Elsevier | es_ES |
dc.subject | Veterinaria | es_ES |
dc.subject.other | Ram | es_ES |
dc.subject.other | Sperm cryopreservation | es_ES |
dc.subject.other | Sperm concentration | es_ES |
dc.subject.other | Sperm quality | es_ES |
dc.subject.other | Fertility | es_ES |
dc.title | Sperm concentration at freezing affects post-thaw quality and fertility of ram semen | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.description.peerreviewed | SI | es_ES |
dc.journal.title | Theriogenology |
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