Show simple item record

dc.contributorFacultad de Ciencias Biologicas y Ambientaleses_ES
dc.contributor.authorOlmo de Medina, Enrique del
dc.contributor.authorBisbal Vigo, Alfonso
dc.contributor.authorMaroto Morales, Alejandro
dc.contributor.authorGarcía Álvarez, Olga
dc.contributor.authorRamón Fernández, Manuel
dc.contributor.authorJiménez Rabadán, Pilar
dc.contributor.authorMartínez Pastor, Felipe 
dc.contributor.authorSoler, Ana J.
dc.contributor.authorGarde López-Brea, Julián
dc.contributor.authorFernández Santos, María Rocío
dc.contributor.otherBiologia Celulares_ES
dc.date2013-04
dc.date.accessioned2019-05-07T10:48:26Z
dc.date.available2019-05-07T10:48:26Z
dc.date.issued2019-05-07
dc.identifier.citationAnimal Reproduction Science, 2013, vol. 138, n. 1–2es_ES
dc.identifier.otherhttps://www.sciencedirect.com/science/article/pii/S0378432013000481#!es_ES
dc.identifier.urihttp://hdl.handle.net/10612/10677
dc.descriptionP. 102-109es_ES
dc.description.abstractThe present study aims to examine the predictive value of some sperm parameters on male fertility. Semen samples from six Manchega rams were collected and cryopreserved. Sperm quality was assessed after thawing and after 2 h of incubation, either in the freezing extender (37 °C) or after dilution in Synthetic Oviductal Fluid (SOF) (38 °C, 5% CO2), attempting to mimic the physiological conditions of the female reproductive tract. The following sperm parameters were evaluated: motility and kinetic parameters by computer-assisted semen analyzer (CASA), and sperm viability (propidium iodide), mitochondrial membrane potential (JC-1), apoptotic-like membrane changes (YO-PRO-1), acrosomal status (PNA-FITC), and intracellular calcium (fluo-3) by flow cytometry. Results showed no significant differences between incubation media neither after thawing nor after incubation. There were no significant correlations between fertility and sperm parameters assessed by flow cytometry. However, after incubation in the freezing extender, sperm samples from males with poor fertility yielded less linearity and velocity (P < 0.05) as indicated by motility parameters analyzed by CASA. These results indicate that kinematic sperm motility parameters evaluation by CASA might be useful to identify samples with poor fertility.es_ES
dc.languageenges_ES
dc.publisherElsevieres_ES
dc.subjectVeterinariaes_ES
dc.subject.otherFertilityes_ES
dc.subject.otherCryopreserved semenes_ES
dc.subject.otherRames_ES
dc.subject.otherFlow cytometryes_ES
dc.subject.otherCASAes_ES
dc.titleFertility of cryopreserved ovine semen is determined by sperm velocityes_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.description.peerreviewedSIes_ES


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record