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    Título
    Trehalose and glycerol have a dose-dependent synergistic effect on the post-thawing quality of ram semen cryopreserved in a soybean lecithin-based extender
    Autor
    Najafi, Abouzar
    Zhandi, Mahdi
    Towhidi, Armin
    Sharafi, Mohsen
    Akbari Sharif, Abbas
    Khodaei Motlagh, Mahdi
    Martínez Pastor, FelipeAutoridad BuleriaORCID
    Facultad/Centro
    Facultad de Ciencias Biologicas y Ambientales
    Área de conocimiento
    Biologia Celular
    Datos de la obra
    Cryobiology, 2013, vol. 66, n. 3
    Editor
    Elsevier
    Fecha
    2013-06
    Abstract
    The objective of this study was to examine the interaction of different concentrations of trehalose [0 (T0), 50 (T50) or 100 (T100) mM] and glycerol [5% (G5) or 7% (G7)] on post-thawed quality of ram semen, cryopreserved in a soybean lecithin (SL)-based extender. Twenty-eight ejaculates were collected from four rams and diluted with six trehalose/glycerol combinations: T0G5, T50G5, T100G5, T0G7, T50G7, and T100G7. Sperm motility (CASA), membrane integrity (eosin/nigrosin) and functionality (HOST), abnormal forms, capacitation status (CTC), mitochondrial activity (rhodamine 123), apoptotic features (Annexin V/propidium iodide) and lipoperoxidation (malondialdehyde production) were evaluated after thawing. Extender T100G5 yielded the highest results for total and progressive motility, sperm velocity, normal morphology, functional membranes, active mitochondria and membrane integrity, with P < 0.05 in general, except for T50G7 (P > 0.05). The combinations T0G5, T0G7 and T100G7 yielded the lowest post-thaw quality. We could not detect significant changes in other kinematic parameters, capacitation status or lipoperoxidation. We conclude that, in our SL-based extender, a combination of 100 mM trehalose and 5% glycerol was the most adequate combination to achieving post-thawing quality in our soybean lecithin-based extender, and our results support that a synergistic effect among trehalose and glycerol exists. We suggest that other combinations could improve these results.
    Materia
    Veterinaria
    Palabras clave
    Ram semen
    Trehalose
    Soybean lecithin
    Cryopreservation
    Glycerol
    Peer review
    SI
    URI
    http://hdl.handle.net/10612/10693
    Versión del editor
    https://www.sciencedirect.com/science/article/pii/S0011224013001090#!
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