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dc.contributorFacultad de Ciencias Biologicas y Ambientaleses_ES
dc.contributor.authorMata Campuzano, María
dc.contributor.authorÁlvarez Rodríguez, Manuel
dc.contributor.authorTamayo Canul, Julio Renan
dc.contributor.authorLópez Urueña, Elena
dc.contributor.authorPaz Cabello, Paulino de
dc.contributor.authorAnel Rodríguez, Luis 
dc.contributor.authorMartínez Pastor, Felipe 
dc.contributor.authorÁlvarez García, Mercedes
dc.contributor.otherBiologia Celulares_ES
dc.date2014-12-30
dc.date.accessioned2019-05-12T23:11:35Z
dc.date.available2019-05-12T23:11:35Z
dc.date.issued2019-05-13
dc.identifier.citationAnimal Reproduction Science, 2014, vol. 151, n. 3–4es_ES
dc.identifier.otherhttps://www.sciencedirect.com/science/article/pii/S0378432014003042es_ES
dc.identifier.urihttp://hdl.handle.net/10612/10697
dc.descriptionP. 137-147es_ES
dc.description.abstractAntioxidants have a potential to improve the quality and fertility of refrigerated-stored ram semen. Reduced glutathione (GSH) and Trolox (0.2, 1 and 5 mM) were evaluated in ram semen preserved at 15 and 5 °C up to 48 and 96 h, respectively. Extenders were also evaluated (15 °C: Tris-citrate-fructose, TCF, without lipids, and TES-Tris-fructose 10% egg yolk, TTF-EY; 5 °C: TTF-EY and 3.5% soybean lecithin, TTF-SL; INRA96 at both temperatures). Storage at 5 °C resulted in poorer quality than 15 °C up to 48 h, while allowing acceptable quality at 96 h. Antioxidants had few effects on sperm quality, with use of Trolox resulting in reduced motility and viability in TCF. Storage at 15 °C in the TCF extender resulted in decreased motility, viability and mitochondrial activity compared with use of TTF-EY. Sperm quality when storage was at 5 °C was similar, but storage in TTF-SL resulted in decreased motility and mitochondrial activity. Acrosomal status was only slightly affected by extender and antioxidant. Mitochondrial activity was improved by antioxidants in TTF-SL, and GSH at 5 mM when semen was stored at 5 °C in TTF-EY. A preliminary artificial insemination trial indicated that supplementation with GSH has the potential for improving lambing (P < 0.1). In conclusion, use of antioxidants resulted in lesser effects than extender composition or storage time on quality of ram semen. Use of Trolox negatively impacted sperm quality and GSH had some positive impacts. The use of soybean lecithin requires further research to assess its impact on mitochondria.es_ES
dc.languageenges_ES
dc.publisherElsevieres_ES
dc.subjectVeterinariaes_ES
dc.subject.otherRames_ES
dc.subject.otherSemenes_ES
dc.subject.otherRefrigerated liquid storagees_ES
dc.subject.otherAntioxidantes_ES
dc.subject.otherExtenderes_ES
dc.titleRefrigerated storage of ram sperm in presence of Trolox and GSH antioxidants: Effect of temperature, extender and storage timees_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.description.peerreviewedSIes_ES


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