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dc.contributorFacultad de Ciencias Biologicas y Ambientaleses_ES
dc.contributor.authorMata Campuzano, María
dc.contributor.authorÁlvarez Rodríguez, Manuel
dc.contributor.authorÁlvarez García, Mercedes
dc.contributor.authorTamayo Canul, Julio Renan
dc.contributor.authorAnel Rodríguez, Luis 
dc.contributor.authorPaz Cabello, Paulino de
dc.contributor.authorMartínez Pastor, Felipe 
dc.contributor.otherBiologia Celulares_ES
dc.date2015-03-01
dc.date.accessioned2019-05-12T23:17:28Z
dc.date.available2019-05-12T23:17:28Z
dc.date.issued2019-05-13
dc.identifier.citationTheriogenology, 2015, vol. 83, n. 4es_ES
dc.identifier.otherhttps://www.sciencedirect.com/science/article/pii/S0093691X14005706es_ES
dc.identifier.urihttp://hdl.handle.net/10612/10700
dc.descriptionP. 520-528es_ES
dc.description.abstractThe performance of cryopreserved semen in ovine artificial insemination still needs improvement. Some antioxidants have been tested, with variable success. We cryopreserved semen from Churra rams using TES-Tris-fructose with 4% glycerol and 10% egg yolk (EY) or 3.5% soybean lecithin (SL), with 1 mM of reduced glutathione (GSH), Trolox, crocin, or cysteamine. Samples were analyzed after thawing and incubation (6 hours, 38 °C) for motility (computer-assisted sperm analysis [CASA]), viability, acrosomal integrity, apoptosis, mitochondrial activity, chromatin status, and lipoperoxidation (malondialdehyde production). Interactions (antioxidant/extender/incubation) were significant for most variables. Extenders yielded similar results, although SL depressed mitochondrial activity and linearity (P < 0.001), it improved motility (P < 0.05), DNA fragmentation (P < 0.05), and acrosomal damage (P < 0.001). The control, GSH, and Trolox showed greater viability with SL (P < 0.01). Cysteamine depressed motility (0 hours: 51.6 ± 2.0% vs. 32.3 ± 4.3%; 6 hours: no motility vs. 32.5 ± 1.9%; P < 0.001), but improved viability when using EY (P = 0.004). Crocin increased acrosomal damage (P = 0.022) but improved linearity-related parameters after thawing (P = 0.014). Trolox considerably reduced malondialdehyde production in both extenders (8.6 ± 0.4 nmol per 108 cells vs. 14.2 ± 0.3 in EY and 20 ± 0.6 in SL; P < 0.001). Interestingly, thiol antioxidants (cysteamine and GSH) increased DNA fragmentation (percentage of DNA fragmentation index), whereas crocin reduced it (P < 0.05). Interactions between extender and antioxidant must be taken into account for improving sperm cryopreservation. Soybean lecithin seems to be a suitable replacement for EY, but its effect on mitochondria must be investigated. Trolox and crocin might be useful for ram semen freezing.es_ES
dc.languageenges_ES
dc.publisherElsevieres_ES
dc.subjectVeterinariaes_ES
dc.subject.otherRames_ES
dc.subject.otherSpermatozoaes_ES
dc.subject.otherAntioxidantes_ES
dc.subject.otherCryopreservationes_ES
dc.subject.otherExtenderes_ES
dc.titlePost-thawing quality and incubation resilience of cryopreserved ram spermatozoa are affected by antioxidant supplementation and choice of extenderes_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.description.peerreviewedSIes_ES


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