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    Título
    Thawing boar semen in the presence of seminal plasma improves motility, modifies subpopulation patterns and reduces chromatin alterations
    Autor
    Fernández Gago, Rocío
    Álvarez Rodríguez, Manuel
    Alonso de la Varga, Marta ElenaAutoridad BuleriaORCID
    González Montaña, José RamiroAutoridad BuleriaORCID
    Alegre Gutiérrez, BeatrizAutoridad Buleria
    Domínguez Fernández de Tejerina, Juan CarlosAutoridad BuleriaORCID
    Martínez Pastor, FelipeAutoridad BuleriaORCID
    Facultad/Centro
    Facultad de Ciencias Biologicas y Ambientales
    Área de conocimiento
    Biologia Celular
    Datos de la obra
    Reproduction, Fertility and Development, 2017, vol. 29 n. 8
    Editor
    CSIRO
    Fecha
    2017
    Abstract
    Seminal plasma could have positive effects on boar semen after thawing. In the present study we investigated changes in the motility and chromatin structure in spermatozoa over 4 h incubation (37°C) of boar semen thawed in the presence of 0%, 10% or 50% seminal plasma from good-fertility boars. Cryopreserved doses were used from seven males, three of which were identified as susceptible to post-thawing chromatin alterations. Motility was analysed by computer-aided sperm analysis every hour, and data were used in a two-step clustering, yielding three subpopulations of spermatozoa (slow non-linear, fast non-linear, fast linear). Chromatin structure was analysed using a sperm chromatin structure assay and flow cytometry to determine the DNA fragmentation index (%DFI) as a percentage, the standard deviation of the DFI (SD-DFI) and the percentage of high DNA stainability (%HDS), indicating chromatin compaction. Thawing without seminal plasma resulted in a rapid loss of motility, whereas seminal plasma helped maintain motility throughout the incubation period and preserved the subpopulation comprising fast and linear spermatozoa. The incidence of chromatin alterations was very low in samples from non-susceptible males, whereas samples from males susceptible to post-thawing chromatin alterations exhibited marked alterations in %DFI and %HDS. Seminal plasma partly prevented these alterations in samples from susceptible males. Overall, 50% seminal plasma was the most efficient concentration to protect motility and chromatin. Some changes were concomitant with physiological events reported previously (e.g., semen thawed with 50% seminal plasma increased the production of reactive oxygen species and yielded higher fertility after AI). Thawing in the presence of seminal plasma could be particularly useful in the case of samples susceptible to post-thawing chromatin damage.
    Materia
    Veterinaria
    Palabras clave
    Cryopreservation
    Flow cytometry
    Sperm physiology
    Peer review
    SI
    URI
    http://hdl.handle.net/10612/10701
    Versión del editor
    http://www.publish.csiro.au/rd/rd15530
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