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dc.contributorFacultad de Ciencias Biologicas y Ambientaleses_ES
dc.contributor.authorNajafi, Abouzar
dc.contributor.authorTaheri, Ramezan Ali
dc.contributor.authorMehdipour, Mahdieh
dc.contributor.authorMartínez Pastor, Felipe 
dc.contributor.authorRouhollahi, Abbas Abbas
dc.contributor.authorNourani, Mohammad Reza
dc.contributor.otherBiologia Celulares_ES
dc.date2019-01-01
dc.date.accessioned2019-05-12T23:43:00Z
dc.date.available2019-05-12T23:43:00Z
dc.date.issued2019-05-13
dc.identifier.citationPoultry Science, 2019, vol. 98, n. 1es_ES
dc.identifier.otherhttps://academic.oup.com/ps/article/98/1/440/5066749es_ES
dc.identifier.urihttp://hdl.handle.net/10612/10721
dc.descriptionP. 440–446es_ES
dc.description.abstractLiposomes could improve the delivery of substances to sperm. This study was conducted to investigate the effect of the antioxidant ellagic acid and ellagic acid-loaded liposomes on post-thawed sperm quality in broiler breeder roosters. Semen was diluted in Beltsville extender containing ellagic acid or ellagic acid-loaded liposomes (ellagic acid at 0 (control), 0.5, 1, and 2 mM) and cryopreserved. Sperm quality was evaluated post-thawing: motility characteristics (Computer-Assisted Semen Analysis), membrane functionality (HOS test), abnormal morphology, mitochondrial activity (Rhodamine 123), apoptotic status (Annexin V/Propidium iodide), malondialdehyde, and antioxidant activities (glutathione peroxidase (GPx), superoxide dismutase (SOD), and total antioxidant capacity (TAC)). The results showed that 1 mM ellagic acid-loaded liposomes improved total motility, membrane functionality, and viability comparing to 0.5 and 2 mM ellagic acid, 2 mM ellagic acid-loaded liposomes, and control group. Mitochondrial activity was significantly higher for 1 mM ellagic acid-loaded liposomes compared to the rest of the treatments, except 1 mM ellagic acid. Ellagic acid at 1 mM in both forms significantly increased GPx and TAC after freeze-thawing (no significant variation for SOD), and also yielded the lower proportion of apoptotic and dead cells. In conclusion, ellagic acid improved post-thawed sperm quality in broiler breeder roosters. The use of liposomes could further enhance the effects of ellagic acid.es_ES
dc.languageenges_ES
dc.publisherOxford University Presses_ES
dc.subjectVeterinariaes_ES
dc.subject.otherEllagic acides_ES
dc.subject.otherLiposomees_ES
dc.subject.otherRoosteres_ES
dc.subject.otherSperm qualityes_ES
dc.subject.otherPost-thawedes_ES
dc.titleImprovement of post-thawed sperm quality in broiler breeder roosters by ellagic acid-loaded liposomeses_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.description.peerreviewedSIes_ES


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