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    Título
    Effect of basic factors of extender composition on post-thawing quality of brown bear electroejaculated spermatozoa
    Autor
    Anel Rodríguez, LuisAutoridad BuleriaORCID
    Gomes Alves, Susana Cláudia
    Álvarez García, MercedesAutoridad BuleriaORCID
    Barragán Santos, Santiago
    Anel Rodríguez, Enrique
    Nicolás, M.
    Martínez Pastor, FelipeAutoridad BuleriaORCID
    Paz Cabello, Paulino deAutoridad BuleriaORCID
    Facultad/Centro
    Facultad de Ciencias Biologicas y Ambientales
    Área de conocimiento
    Biologia Celular
    Datos de la obra
    Theriogenology, 2010, vol. 74, n. 4
    Editor
    Elsevier
    Fecha
    2010-09-01
    Abstract
    The improvement of freezing extenders is critical when defining sperm cryopreservation protocols for wild species, in order to create germplasm banks. The aim of this study was to evaluate the effect of additives (Equex Paste and EDTA) supplementation, egg-yolk (10 and 20%) and glycerol (4 and 8%) concentrations and extender osmolality (300 and 320 mOsm/kg) on the post-thawing quality of brown bear semen. Semen was obtained from 20 adult males by electroejaculation, and centrifugated individually (600 × g for 6 min). The pellets were diluted 1:1 in the corresponding extender TTF (TES-Tris-Fructose with the aforementioned variants) and cooled to 5 °C. Then, it was diluted down to 100 × 106 spz/mL, loaded in 0.25 mL straws and frozen at −20°C/min. After thawing (in water at 65 °C for 6s), the semen samples were assessed for motility (CASA), viability (SYBR-14 with propidium iodide), acrosomal status (PNA-FITC with propidium iodide) and mitochondrial activity (JC-1). Extender supplementation with additives rendered significantly higher results for these sperm parameters. Comparing the two percentages of egg yolk, 20% egg yolk showed the highest motility results, percentages of viable spermatozoa and viable spermatozoa with intact acrosome. No differences were detected among samples frozen using 4 or 8% glycerol. For extender osmolality, 300 mOsm/kg showed higher values of VAP, VCL, VSL, and ALH than 320 mOsm/kg. Based on the best performance of sperm motility, viability and acrosome status, we conclude that the most suitable extender to cryopreserve brown bear spermatozoa was TTF adjusted to 300 mOsm/kg, supplemented with 20% egg yolk, 4–8% glycerol, and the additives 1% Equex paste and 2% EDTA.
    Materia
    Veterinaria
    Palabras clave
    Brown bear
    Cryopreservation
    Spermatozoa
    Extender
    Germplasm banks
    Peer review
    SI
    URI
    http://hdl.handle.net/10612/10744
    Versión del editor
    https://www.sciencedirect.com/science/article/pii/S0093691X10001500
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