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dc.contributorFacultad de Ciencias Biologicas y Ambientaleses_ES
dc.contributor.authorNajafi, Abouzar
dc.contributor.authorNajafi, Mir Hossein
dc.contributor.authorZanganeh, Zaynab
dc.contributor.authorSharafi, Mohsen
dc.contributor.authorMartínez Pastor, Felipe 
dc.contributor.authorAdeldust, Hamideh M.
dc.contributor.otherBiologia Celulares_ES
dc.date2014-12
dc.date.accessioned2019-05-16T10:46:30Z
dc.date.available2019-05-16T10:46:30Z
dc.date.issued2019-05-16
dc.identifier.citationReproduction in Domestic Animals, 2014, vol. 49, n. 6es_ES
dc.identifier.otherhttps://onlinelibrary.wiley.com/doi/full/10.1111/rda.12405es_ES
dc.identifier.urihttp://hdl.handle.net/10612/10757
dc.descriptionP. 934-940es_ES
dc.description.abstractA soybean lecithin‐based extender supplemented with hyaluronic acid (HA) was assayed for effectiveness to improve the quality of frozen–thawed ram semen. HA has not been tested yet in an extender containing soybean lecithin for freezing ram semen. Thus, the aim of this study was to analyse the effects of soybean lecithin at 1% or 1.5% along with HA at 0, 0.5 and 1 mg ml‐1 in a Tris‐based extender on the motion characteristics, membrane integrity (HOST), viability, GSH peroxidase (GSH‐PX) activity, lipid peroxidation and acrosomal status after freezing–thawing. Semen was collected from four Mehraban rams during the breeding season and frozen in the six lecithin×HA extenders. The extender containing 1.5% lecithin supplemented with no HA yielded higher total motility (52.5%±1.6), viability (55.8%±1.6) and membrane integrity (44.5%±1.7), but the effects of the lecithin concentration did not reach signification. Linearity‐related parameters, ALH, BCF, lipid peroxidation, GSH‐PX activity, morphology and acrosomal status were not affected by the extender composition. In general, adding HA significantly decreased sperm velocity (1 mg ml‐1 HA), total motility (only with 1.5% lecithin), viability (1 mg ml‐1 HA for 1% lecithin; both concentrations for 1.5% lecithin) and membrane integrity. In conclusion, adding HA to the freezing extender supplemented with soybean lecithin failed to improve quality‐related variables in ram semen. Increasing the lecithin content could have a positive effect, but further studies are needed.es_ES
dc.languageenges_ES
dc.publisherJohn Wiley & Sonses_ES
dc.subjectVeterinariaes_ES
dc.subject.otherCriopreservaciónes_ES
dc.subject.otherGanado ovinoes_ES
dc.subject.otherSemenes_ES
dc.subject.otherLecitinaes_ES
dc.subject.otherÁcido hialurónicoes_ES
dc.subject.otherAntioxidanteses_ES
dc.titleCryopreservation of Ram Semen in Extenders Containing Soybean Lecithin as Cryoprotectant and Hyaluronic Acid as Antioxidantes_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.description.peerreviewedSIes_ES


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