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    Título
    Effects on brown bear (Ursus arctos) spermatozoa freezability of different extender and dilution ratios used for pre-freezing centrifugation
    Autor
    Nicolás, M.
    Álvarez García, MercedesAutoridad BuleriaORCID
    Gomes Alves, Susana Cláudia
    Mata Campuzano, María
    Barragán Santos, Santiago
    Martínez Pastor, FelipeAutoridad BuleriaORCID
    Paz Cabello, Paulino deAutoridad BuleriaORCID
    Anel Rodríguez, LuisAutoridad BuleriaORCID
    Facultad/Centro
    Facultad de Ciencias Biologicas y Ambientales
    Área de conocimiento
    Biologia Celular
    Datos de la obra
    European Journal of Wildlife Research, 2011, vol. 57, n. 2
    Editor
    Springer
    Fecha
    2011-04
    Abstract
    The objective of this study was to determine how the extender and dilution ratio used during centrifugation affect bear spermatozoa quality before and after freezing–thawing. Semen was collected from 15 brown bears by electroejaculation. In experiment 1, semen was divided into five aliquots and diluted using one of the following extenders: Tris-citric-glucose (TCG), Tris-citric-glucose-3% BSA, Tris-citric-glucose-1% egg yolk or CaninePro. In experiment 2, semen was divided into five aliquots and diluted 1:1, 1:4, 1:8 or 1:16 (semen:extender) with Tris-citric-glucose. In both experiments, one aliquot was left undiluted and it was used as a control. All the aliquots were centrifuged at 600×g for 6 min and frozen. Samples were analysed by post-thawing for motility (CASA) and, by flow cytometry, for viability (YO-PRO-1), acrosomal status (PNA-FITC/PI) and mitochondrial status (JC-1). CaninePro rendered the highest motility with respect to the undiluted control (total motility, 53.1% vs. 38.5%, P < 0.001), and CaninePro and TCG significantly increased the percentage of viable and acrosome-intact spermatozoa (43.2 and 43.4, respectively, vs. 39.4, P < 0.05). In experiment 2, dilution 1:4 yielded the highest value of total motility (78.8 vs. 67.2, P < 0.05) and proportion of spermatozoa with intact membrane and acrosome (64.5 vs. 54.4, P < 0.01). In general, diluting 1:4 or 1:8 brown bear semen prior to centrifugation improved the motility and acrosome status of the thawed spermatozoa.
    Materia
    Veterinaria
    Palabras clave
    Brown bear
    Spermatozoa
    Centrifugation
    Extender
    Dilution
    URI
    http://hdl.handle.net/10612/10769
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    50 Effects on brown bear (Ursus arctos) EurJWildlRes_057_0259_2011.pdf
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