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    Título
    Evaluation of ram semen quality using polyacrylamide gel instead of cervical mucus in the sperm penetration test
    Autor
    Martínez Rodríguez, Carmen
    Álvarez García, MercedesAutoridad Buleria ORCID
    Ordás, L.
    Chamorro Álvarez, César ÁngelAutoridad Buleria ORCID
    Martínez Pastor, FelipeAutoridad Buleria ORCID
    Anel Rodríguez, LuisAutoridad Buleria ORCID
    Paz Cabello, Paulino deAutoridad Buleria ORCID
    Facultad/Centro
    Facultad de Ciencias Biologicas y Ambientales
    Área de conocimiento
    Biologia Celular
    Datos de la obra
    Theriogenology, 2012, vol. 77, n. 8
    Editor
    Elsevier
    Fecha
    2012-05
    Descripción física
    P. 1575-1586
    Abstract
    Fertility is a very complex biological function that depends on several properties of the spermatozoa, including sperm motility. Two objectives are analyzed in this study: (1) Replace the cervical mucus by a synthetic medium in a sperm penetration test, and (2) evaluating the results of this test objectively analyzing the sperm number that migrates. In experiment 1, we have tested eight concentrations of acrylamide (1%–2%). Rheological properties of media were analyzed. The plastic straws, loaded with acrylamide, were placed vertically on the semen sample tube for 15 min at 39 °C. After, the acrylamides were placed, by segments of 5 mm, into wells of a 24-well plate, dyed with Hoechst 33342 and the number of spermatozoa were calculated by automated microscopy analysis. The 1.55% and 1.6% acrylamide gel showed a number of spermatozoa emigrating closer to that seen with natural mucus. In experiment 2, we applied the sperm penetration in acrylamide 1.6% and 1.55% using fresh semen and cooled semen at 15 °C and 5 °C. The spermatozoa counts were performed for each segment of 10 mm. Semen chilled at 15 °C presented intermediate values of sperm counts in comparison with fresh semen (higher) and 5 °C chilled semen. The sperm counts do not differ between acrylamides but the rheological properties of acrylamide 1.6% were more similar to those of the natural cervical mucus. In experiment 3, we have observed significant correlations between the number of spermatozoa and several sperm quality parameters (positive: progressive motility and velocity according to the straight path; negative: damaged acrosomes and apoptotic cells) in 1.6% acrylamide media. We conclude that the size of the cell subpopulation, objectively calculated, that migrate beyond 20 mm in 0.5-mL straws filled with acrylamide is a useful parameter in ram sperm quality assessment and further studies are needed to evaluate its relationship with field fertility.
    Materia
    Veterinaria
    Palabras clave
    Ram
    Sperm quality
    Penetration test
    Motility
    Mucus
    Idioma
    eng
    Tipo documental
    info:eu-repo/semantics/article
    Peer review
    SI
    URI
    http://hdl.handle.net/10612/10770
    Versión del editor
    https://www.sciencedirect.com/science/article/pii/S0093691X1100625X#!
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